The simultaneous determination of furazolidone, sodium nifurstyrate, nifurpirinol and panazon was studied. Nitrofurans were extracted with methanol-N, N-′ dimethylformamide (49:1) and filtered. The filtrate was concentrated and to high performance liquid chromatography (HPLC) a 380nm ultraviolet-visible spectrophotometric detector. The recoveries of furazolidone, sodium nifurstyrate, nifurpirinol and panazon added to cultured fishes at the 0.4ppm level were in the ranges of 93-101%, 75-98%, 81-100% and 54-72%, respectively. The limits of detection were 0.05ppm for furazolidone, sodium nifurstyrate, nifurpirinol and 0.1ppm for panazon.