In the previous report we conld confirm as the result of our research that the T. T. C. method published by Neal and Calbert was practically applicable to detecting antibiotics contained in milk, and made public a new test procedure for the same purpose in modification of this method.
In this report we tried to determine whether the widely used reagents, methylene blue and resazurine, could replace the conventional one, T. T. C., used in the above-mentioned methods. Judging from our experimental results obtained, it was made clear that the both reagents were usable in replacement of T. T. C. and among them the application of resazurine was less time-consuming and more practical than T. T. C.