Cancer is one of the diseases that rapidly appear worldwide, affecting 82% of the world's population. It is a complex disorder involving modifications in the body's physiological conditions. This disease is considered a global issue, primarily affecting developing countries. According to research surveys, 63% of cancer-related deaths are reported in developing countries. Some studies describe it as an abnormal condition where a group of physiological cells ignore cell divisions and grow uncontrollably. These cells do not respond to signals that activate the natural cell cycle, exhibiting a degree of self-sufficiency leading to unregulated growth and spread in transformed cells. 90% of cancer cell spread is fatal due to their migration to adjacent tissues.

Objectives: Study the effect of 5-Fluorouracil drug and Annona muricata seed extract on chemically induced tumors by Thioacetamide on the livers of laboratory rats and determine the changes occurring in the tissues.

Method of work: Taking (48) male rats and dividing them into eight groups, each group consisting of six animals. (The first group) the control group, the natural control group, for a period of 28 days. (The second group) treated with 5-fluorouracil once a week for 28 days. (The third group) treated with the plant extract of seeds for 28 days. (The fourth group) treated with the plant extract of seeds and 5-fluorouracil for 28 days. (The fifth group) received daily doses of thioacetamide for 5 days. (The sixth group) received daily doses of thioacetamide for 5 days, then treated with 5-fluorouracil for 23 days as mentioned above. (The seventh group) received daily doses of thioacetamide and then treated with the extract. (The eighth group) received daily doses of thioacetamide for 5 days and then treated with 5-fluorouracil and the plant extract of seeds for 23 days.

Results and Conclusion: Histological sections of the liver and chemical tests showed clear positive effects of the plant extract of seeds in rats treated with thioacetamide. Positive effects were also observed in rats treated with thioacetamide and injected with 5-Fluorouracil, and in rats treated with thioacetamide and the plant extract of seeds along with 5-Fluorouracil.