Mastitis, an inflammation of the mammary gland. Staphylococcus aureus is the most common cause of economic loss in dairy cattle due to decrease quality and quantity of milk. This study was aimed to find-out the prevalence of S.aureus in case of bovine mastitis (clinical) through conventional method. Absolute diagnosis of pathogens plays great role in cure of disease. A total of 27 S. aureus isolates were isolated from 50 individual milk samples from dairy cows with clinical mastitis. The results showed that they were positive in gram staining (forming a bacteria cluster of purple cocci), catalase test, coagulase test, blood agar haemolysis, and DNase test. The mannitol, trehalose, sucrose, maltose, and lactose tests were also positive. The prevalence of bacterial isolates in conventional detection were 55%.