Filamentous gemmae produced at the tips of chloronema cells emerging from both rhizoids from stem epidermal cells and leaf costa cells were newly reported from a single population of Scopelophila ligulata in Kyoto Prefecture, central Japan. They are composed of 4 to 5 cells including a slightly differentiated basal cell. In addition, protonemal regeneration was also observed both in S. ligulata and S. cataractae.