NON-SELECTIVE EXPRESSION OF SHORT-WAVELENGTH CONE OPSIN IMPROVES LEARNING IN MICE WITH RETINAL DEGENERATION IN A VISUALLY GUIDED TASK

O. S. Idzhilova; Иджилова О. С.; D. E. Kolotova; Колотова Д. Е.; G. R. Smirnova; Смирнова Г. Р.; A. Abonakour; Абонакур А.; D. A. Dolgikh; Долгих Д. А.; L. E. Petrovskaya; Петровская Л. Е.; Academician of the RAS M. P. Kirpichnikov; Кирпичников М. П.; Academician of the RAS M. A. Ostrovsky; Островский М. А.; A. Yu. Malyshev; Малышев А. Ю.

doi:10.31857/S268673892360005X

NON-SELECTIVE EXPRESSION OF SHORT-WAVELENGTH CONE OPSIN IMPROVES LEARNING IN MICE WITH RETINAL DEGENERATION IN A VISUALLY GUIDED TASK

Authors: Idzhilova O.S.¹^,2, Kolotova D.E.¹^,2, Smirnova G.R.², Abonakour A.¹^,3, Dolgikh D.A.²^,4^,5, Petrovskaya L.E.⁴, Kirpichnikov A.o.⁴^,5, Ostrovsky A.o.²^,5, Malyshev A.Y.¹
Affiliations:
1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences
2. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
3. Moscow Institute of Physics and Technology (National Research University)
4. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
5. Biological Faculty, Moscow State University
Issue: Vol 510, No 1 (2023)
Pages: 297-302
Section: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/135701
DOI: https://doi.org/10.31857/S268673892360005X
EDN: https://elibrary.ru/QHNHBW
ID: 135701

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Abstract

Here, we explore the potential of an animal opsin non-selectively expressed in various neuronal elements of the degenerative retina to restore impaired visual function. In this study, a knockout murine model of inherited retinal distrophy was used. Animals were injected intravitreally with either a virus carrying gene of the short-wavelength cone opsin associated with a reporter fluorescent protein, or a control virus carrying the sequence of a modified fluorescent protein that had an enhanced membrane tropism. The viral transduction induced pronounced opsin expression in ganglion, bipolar, and horizontal retinal neurons. Behavioral testing of the treated mice in the visually guided task in the trapezoid Morris water maze showed a partial recovery of learning ability in animals whose retinas had been transduced with the cone opsin.

Keywords

optogenetic prosthetics, retinal degeneration, adeno-associated viruses, cone opsin

References

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