On Particular Regimens of Derivative UV-spectrophotometry for Comparative Analysis of Proteins

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

Bovine serum albumin and two oxygen transport proteins, hemocyanin from the snail Achatina fulica and bovine hemoglobin, were used to define what regimens of derivative UV-spectrophotometry are most appropriate for using it as an express technique for nondestructive comparative analysis of native proteins preparations. It was found that the fourth derivatives of proteins absorption spectra make it possible to detect the individual bands of aromatic amino acids in a way optimal for solving certain practical problems. An algorithm for calculating the fourth derivatives was selected experimentally. To verify the approach, the fourth derivatives of the native proteins spectra were reconstructed by combining those of individual aromatic amino acids spectra in the range of 240–300 nm. To demonstrate the individual differences between proteins, it is proposed to use the correlation coefficients of the fourth derivatives of spectra in the range of 240–300 nm or in the wavelength range of tyrosine and tryptophan. Although this approach does not provide for estimating the exact contents of individual aromatic amino acids in proteins, it allows comparing different proteins between each other. The proposed approach makes it possible to obtain an individual spectral “portrait” of a protein, which distinguishes it from other proteins and is useful as a reference for further experimental work with it.

About the authors

A. Y. Lianguzov

St. Petersburg State University

Author for correspondence.
Email: andrey.lyanguzov@spbu.ru
Russia, 199034, St. Petersburg

N. M. Malygina

St. Petersburg State University; Kirov Military Medical Academy

Email: andrey.lyanguzov@spbu.ru
Russia, 199034, St. Petersburg; Russia, 194044, St. Petersburg

A. M. Ivanov

Kirov Military Medical Academy

Email: andrey.lyanguzov@spbu.ru
Russia, 194044, St. Petersburg

T. A. Petrova

St. Petersburg State University

Email: andrey.lyanguzov@spbu.ru
Russia, 199034, St. Petersburg

References

  1. Petrova T.A., Lyanguzov A.Yu., Malygina N.M. // J. Evol. Biochem. Physiol. 2016. V. 52. P. 37–45.
  2. R Project. Reference date: 21.06.2022. http://cran.r-project.org
  3. Mathews J.H. // Int. J. Math. Educ. Sci. Technol. 2003. V. 34. P. 280–287.
  4. Decker H., Hellmann N., Jaenicke E., Lieb B., Meissner U., Markl J. // Integr. Comp. Biol. 2007. V. 47. P. 631–644.
  5. Nelson D.L., Cox M.M. Lehninger Principles of Biochemistry. 7th Edition, N.Y., W.H. Freeman and Company, 2017. 3270 p.
  6. Peters T. Jr. All about Albumin. Biochemystry, Genetics and Medical Applications. Academic Press. 1995. 432 p.
  7. EL-Sharif H.F., Aizawa H., Reddy S.M. // Sens. Actuators, B. 2015. V. 206. P. 239–245.
  8. van Holde K.E., Miller K.I., Decker H. // J. Biol. Chem. 2001. V. 276. P. 15563–15566.
  9. Markl J. // Biochim. Biophys. Acta. 2013. V. 1834. P. 1840–1852.
  10. Blanc L., Papoin J., Debnath G., Vidal M., Amson R., Telerman A., An X., Mohandas N. // Am. J. Hematol. 2015. V. 90. P. 235–241.
  11. Fox D.L. Biochromy of the Mollusca. in The Mollusca. V. 2. Environmental Biochemistry and Physiology / Ed. Hochachka P.W. N.Y., Academic Press, 1983. 362 p.
  12. Boysen R.I., Hearn M.T.W. //Amino Acids, Peptides and Proteins in Organic Chemistry. V. 5. Analysis and Function of Amino Acids and Peptides. / Ed. by B. Hughes A.B., Weinheim, Wiley-VCH, 2012. 508 p.
  13. Talsky G. Derivative spectrophotometry / Ed. T. Mager. Weinheim, Wiley, John & Sons, 1994. 228 p.
  14. Lavrinenko I.A., Vashanov G.A., Ruban M.K. // J. Appl. Spectrosc. 2014. V. 80. P. 899–904.
  15. Ojeda C.B., Rojas F.S. Recent applications in derivative ultraviolet/visible absorption spectrophotometry: 2009–2011: a review // Microchem. J. 2013. V. 106. P. 1–16.
  16. The UniProt Consortium. UniProt: the Universal Protein Knowledgebase in 2021 // Nucleic Acids Res. 2021. V. 49. P. D480–D489.

Supplementary files

Supplementary Files
Action
1. JATS XML
2.

Download (157KB)
3.

Download (145KB)
4.

Download (439KB)
5.

Download (234KB)

Copyright (c) 2023 А.Ю. Лянгузов, Н.М. Малыгина, А.М. Иванов, Т.А. Петрова

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies