Generation of a Null Mutation of the  toothrin  Gene by Targeted Homologous Recombination in  Drosophila melanogaster

E. E. Kuvaeva; Куваева Е. Е.; D. A. Kulikova; Куликова Д. А.; O. B. Simonova; Симонова О. Б.; I. B. Mertsalov; Мерцалов И. Б.

doi:10.31857/S0475145023030059

Generation of a Null Mutation of the toothrin Gene by Targeted Homologous Recombination in Drosophila melanogaster

Authors: Kuvaeva E.E.¹, Kulikova D.A.¹, Simonova O.B.¹, Mertsalov I.B.¹
Affiliations:
1. Koltzov Institute of Developmental Biology of Russian Academy of Sciences
Issue: Vol 54, No 3 (2023)
Pages: 232-238
Section: Short communications
URL: https://journals.rcsi.science/0475-1450/article/view/137061
DOI: https://doi.org/10.31857/S0475145023030059
EDN: https://elibrary.ru/ZRSGJG
ID: 137061

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Abstract
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Abstract

Drosophila toothrin gene is related to the conserved d4 family of genes that encode specific transcription factors. In early works, we investigated the effect of tth gene overexpression and characterized the specific pattern of its expression in the nervous system, but mutations in the tth gene have not yet been found. Null mutants are essential for analyzing gene function and screening of partner genes. Here we report the generation of the first tth gene knockout in Drosophila using a homologous recombination technique.

Keywords

d4 gene family, toothrin, gene knockout, Drosophila melanogaster

References

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