Reversible Increase in Resistance of A-431 Carcinoma Cells to TRAIL-Induced Apoptosis in Confluent Cultures Corresponds to a Decrease in Expression of DR4 and DR5 Receptors

R. S. Fadeev; Фадеев Р. С.; N. V. Dolgikh; Долгих Н. В.; A. V. Chekanov; Чеканов А. В.; A. S. Senotov; Сенотов А. С.; K. S. Krasnov; Краснов К. С.; M. I. Kobyakova; Кобякова М. И.; Ya. V. Lomovskaya; Ломовская Я. В.; I. S. Fadeevа; Фадеева И. С.; V. S. Akatov; Акатов В. С.

doi:10.31857/S0233475523020020

Reversible Increase in Resistance of A-431 Carcinoma Cells to TRAIL-Induced Apoptosis in Confluent Cultures Corresponds to a Decrease in Expression of DR4 and DR5 Receptors

Authors: Fadeev R.S.¹^,2, Dolgikh N.V.¹, Chekanov A.V.¹, Senotov A.S.¹, Krasnov K.S.¹^,2, Kobyakova M.I.¹, Lomovskaya Y.V.¹, Fadeevа I.S.¹^,2, Akatov V.S.¹^,2
Affiliations:
1. Institute for Theoretical and Experimental Biophysics, Russian Academy of Sciences
2. Puschino State Natural Science Institute
Issue: Vol 40, No 2 (2023)
Pages: 105-111
Section: Articles
URL: https://journals.rcsi.science/0233-4755/article/view/135012
DOI: https://doi.org/10.31857/S0233475523020020
EDN: https://elibrary.ru/LALOSK
ID: 135012

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Abstract

TRAIL (TNF alpha Related Apoptosis Inducing Ligand) cytokine is of great interest for the development of targeted antitumor drugs. We have previously found a reversible increase in tumour cell resistance to TRAIL-induced apoptosis in confluent cultures. In this work we show that increase in resistance of A-431 cells to TRAIL-induced death in confluent culture is associated with reduced expression of pro-apoptotic receptors DR4 and DR5 with absence of anti-apoptotic receptors DcR1 and DcR2 on cell surface. Decreased representation of DR4 and DR5 receptors on the cell surface is accompanied by a lack of activation of the pro-apoptotic protein Bid, effector caspase 3 under the action of recombinant protein izTRAIL, which leads to an increase in TRAIL resistance. Our results indicate that reversible increase in resistance of human carcinoma A-431 cells to TRAIL-induced apoptosis in confluent cultures is caused by decrease in expression of DR4 and DR5 receptors on cell surface.

Keywords

human carcinoma cells, TRAIL-induced apoptosis, confluent culture, TRAIL resistance, TRAIL receptors

References

Chamuleau M.E., Ossenkoppele G.J., van Rhenen A., van Dreunen L., Jirka S.M., Zevenbergen A., Schuurhuis G.J., van de Loosdrecht A.A. 2011. High TRAIL-R3 expression on leukemic blasts is associated with poor outcome and induces apoptosis-resistance which can be overcome by targeting TRAIL-R2. Leuk. Res. 35, 741–749.
Cheng J., Hylander B.L., Baer M.R., Chen X., Repasky E.A. 2006. Multiple mechanisms underlie resistance of leukemia cells to Apo2 Ligand/TRAIL. Mol. Cancer Ther. 5, 1844–1853.
Riccioni R., Pasquini L., Mariani G., Saulle E., Rossini A., Diverio D., Pelosi E., Vitale A., Chierichini A., Cedrone M., Foa R., Coco F., Peschle C., Testa U. 2005. TRAIL decoy receptors mediate resistance of acute myeloid leukemia cells to TRAIL. Haematologica. 90, 612–624.
Buchsbaum D.J., Zhou T., Lobuglio A.F. 2006.TRAIL receptor-targeted therapy. Future Oncol. 2, 493–508.
Zhang L., Fang B. 2005. Mechanisms of resistance to TRAIL-induced apoptosis in cancer. Cancer Gene Therapy. 12, 228–237.
Grayson K.A., Jyotsana N., Ortiz-Otero N., King M.R. 2011. Overcoming TRAIL-resistance by sensitizing prostate cancer 3D spheroids with taxanes. PLoS One. 16 (3), e0246733.
Chandrasekaran S., Marshall J.R., Messing J.A., Hsu J.W., King M.R. 2014. TRAIL-mediated apoptosis in breast cancer cells cultured as 3D spheroids. PLoS One. 9 (10), e111487.
Фадеев P.C., Чеканов А.В., Долгиx Н.В., Акатов В.С. 2012. Повышение резистентности опухолевых клеток линии А431 к TRAIL-индуцированному апоптозу в конфлюэнтных культурах. Биофизика. 57 (4), 649–654.
Долгих Н.В., Чеканов А.В., Фадеев Р.С., Акатов В.С. 2017. Различия в активации сигнальных путей опухолевых клеток А431 при конфлюэнт-зависимой и конфлюэнт-независимой TRAIL-резистентности. Биол. мембраны. 34 (6), 124–136.
Kobyakova M., Lomovskaya Y., Senotov A., Lomovsky A., Minaychev V., Fadeeva I., Shtatnova D., Krasnov K., Zvyagina A., Odinokova I., Akatov V., Fadeev R. 2022. The increase in the drug resistance of acute myeloid leukemia THP-1 cells in high-density cell culture is associated with inflammatory-like activation and anti-apoptotic Bcl-2 proteins. Int. J. Mol. Sci. 23, 7881.
Lomovskaya Y.V., Kobyakova M.I., Senotov A.S., Lomovsky A.I., Minaychev V.V., Fadeeva I.S., Shtatnova D.Y., Krasnov K.S., Zvyagina A.I., Akatov V.S., Fadeev R.S. 2022. Macrophage-like THP-1 cells derived from high-density cell culture are resistant to TRAIL-induced cell death via down-regulation of death-receptors DR4 and DR5. Biomolecules. 12, 150.
Suzuki K., Hiroaki H., Kohda D., Tanaka T. 1998. An isoleucine zipper peptide forms a native-like triple stranded coiled coil in solution. Protein Eng. 11, 1051–1055.
Fadeev R., Chekanov A., Solovieva M., Bezborodova O., Nemtsova E., Dolgikh N., Fadeeva I., Senotov A., Kobyakova M., Evstratova Y., Yakubovskaya R., Akatov V. 2019. Improved anticancer effect of recombinant protein izTRAIL combined with sorafenib and peptide iRGD. Int. J. Mol. Sci. 20, 525.
Lomovsky A., Baburina Y., Odinokova I., Kobyakova M., Evstratova Y., Sotnikova L., Krestinin R., Krestinina O. 2020. Melatonin can modulate the effect of navitoclax (ABT-737) in HL-60 cells. Antioxidants. 9, 1143.
Lavrik I.N., Krammer P.H. 2012. Regulation of CD95/Fas signaling at the DISC. Cell Death Differ. 19 (1), 36–41.
Galluzzi L., Maiuri M., Vitale I., Zischka H., Castedo M., Zitvogel L., Kroemer G. 2007. Cell death modalities: Classification and pathophysiological implications. Cell Death Differ. 14 (7), 1237–1243.
Waterhouse N., Sedelies K., Sutton V. Pinkoski M., Thia K., Johnstone R., Bird P., Green D., Trapani J. 2006. Functional dissociation of ΔΨm and cytochrome c release defines the contribution of mitochondria upstream of caspase activation during granzyme B-induced apoptosis. Cell Death Differ. 13, 607–618.
Zou H., Henzel W., Liu X., Lutschg A., Wang X. 1997. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell. 90 (3), 405–413.
Dida F., Li Y., Iwao A., Deguchi T., Azuma E., Komada Y. 2008. Resistance to TRAIL-induced apoptosis caused by constitutional phosphorylation of Akt and PTEN in acute lymphoblastic leukemia cells. Exp. Hematology. 36 (10), 1343–1353.