Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis

R. A. Miftakhov; Мифтахов Р. А.; T. V. Nasedkina; Наседкина Т. В.; A. S. Zasedatelev; Заседателев А. С.; V. E. Shershov; Шершов В. Е.; V. E. Kuznetsova; Кузнецова В. Е.; A. I. Levashova; Левашова А. И.; S. A. Lapa; Лапа С. А.; V. A. Vasiliskov; Василисков В. А.; A. Yu. Ikonnikova; Иконникова А. Ю.; A. V. Chudinov; Чудинов А. В.

doi:10.31857/S0132342323050044

Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis

Authors: Miftakhov R.A.¹, Nasedkina T.V.¹, Zasedatelev A.S.¹, Shershov V.E.¹, Kuznetsova V.E.¹, Levashova A.I.¹, Lapa S.A.¹, Vasiliskov V.A.¹, Ikonnikova A.Y.¹, Chudinov A.V.¹
Affiliations:
1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Issue: Vol 49, No 6 (2023)
Pages: 641-648
Section: ПИСЬМА РЕДАКТОРУ
URL: https://journals.rcsi.science/0132-3423/article/view/148163
DOI: https://doi.org/10.31857/S0132342323050044
EDN: https://elibrary.ru/ARRVXQ
ID: 148163

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Abstract
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Abstract

A method for manufacturing biochips by photolithography with hydrogel cells made of brush copolymers based on acrylic acid and acrylamide, fixed at one end on the surface of a polymer substrate has been developed. Hydrogel cells with reactive carboxyl groups were used for covalent immobilization of oligonucleotide probes. The effectiveness of the method was demonstrated in the hybridization analysis of DNA by targets of different lengths of the sequence site 7 of the exon of the human ABO gene.

Keywords

biochips, brush polymers, immobilization of oligonucleotides, DNA hybridization analysis

References

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