Alternative Chromophore Binding Sites in Non-Covalent Fluorescent Complexes of Bacterial Lipocalin
- Authors: Goryacheva E.A.1, Artemyev I.V.1, Arhipova S.F.1, Rossohin A.V.2, Gilvanov A.R.1, Pletnev V.Z.1, Pletnevа N.V.1
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Research Center of Neurology
- Issue: Vol 49, No 5 (2023)
- Pages: 548-552
- Section: ПИСЬМА РЕДАКТОРУ
- URL: https://journals.rcsi.science/0132-3423/article/view/139263
- DOI: https://doi.org/10.31857/S0132342323050020
- EDN: https://elibrary.ru/AAZYGK
- ID: 139263
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Abstract
The three-dimensional structure of DiB3, a fluorescent non-covalent complex of a genetically engineered variant of the bacterial protein lipocalin Blc with a synthetic GFP-like chromophore M739, was determined by the calculation method of molecular mechanics. Compared to the crystal structure of the related DiB1 complex, an alternative binding site for the M739 chromophore was found in the structure of the DiB3 complex.
About the authors
E. A. Goryacheva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
I. V. Artemyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
S. F. Arhipova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
A. V. Rossohin
Research Center of Neurology
Email: goryacheva@ibch.ru
Russia, 105064, Moscow, per. Obuha 5
A. R. Gilvanov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
V. Z. Pletnev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
N. V. Pletnevа
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10
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