Molecular Marking in  Brassica oleracea  L. Breeding for Resistance to Fusarium Wilt

E. V. Dubina; Дубина Е. В.; Yu. A. Makukha; Макуха Ю. А.; A. M. Artem’eva; Артемьева А. М.; D. A. Fateev; Фатеев Д. А.; S. V. Garkusha; Гаркуша С. В.; O. L. Gorun; Горун О. Л.; S. A. Lesnyak; Лесняк С. А.

doi:10.31857/S001667582310003X

Molecular Marking in Brassica oleracea L. Breeding for Resistance to Fusarium Wilt

Authors: Dubina E.V.¹^,2, Makukha Y.A.¹, Artem’eva A.M.³, Fateev D.A.³, Garkusha S.V.¹, Gorun O.L.¹, Lesnyak S.A.¹
Affiliations:
1. Federal Scientific Rice Centre
2. Kuban State Agrarian University
3. Federal Research Centre Vavilov All-Russian Institute of Plant Genetic Resources
Issue: Vol 59, No 10 (2023)
Pages: 1134-1141
Section: ГЕНЕТИКА РАСТЕНИЙ
URL: https://journals.rcsi.science/0016-6758/article/view/140387
DOI: https://doi.org/10.31857/S001667582310003X
EDN: https://elibrary.ru/ZTYMZI
ID: 140387

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

This article presents the results of research on the identification of informative DNA marker systems, providing reliable control of the resistance gene to fusarium wilt Foc1 presence in white cabbage breeding material. At the beginning of the work 14 molecular markers, taken from VegMarks database and literature sources were tested on isogenic white cabbage lines with contrasting resistance to fusarium wilt (resistant line DT-46 and susceptible line Kb1P). InDel-marker M10 and SSR-markers Frg13 and O110-D01 have been ascertained to show polymorphism between white cabbage forms with contrasting resistance to fusarium wilt. Also PCR-analysis of segregating F₂-population plants of hybrid combination DT-46 × Kb1P using these markers and phytopathology testing have been conducted. As a result of statistical analysis of segregation it has been found that SSR-marker Ol10-D01 only is cosegregated with the trait of resistance to fusarium wilt as expected segregation of F₂-plants by genotype 1 : 2 : 1 according to Mendel’s law, the least recombination frequency between the resistance gene Foc1 and the marker (1.6%) has just been observed by this locus only and highly polymorphic (PIC = 0.51).

Keywords

white cabbage, fusarium wilt, SSR-marker, PCR-analysis, segregating population.

References

Pu Z., Shimizu M., Zhang Y. et al. Genetic mapping of a fusarium wilt resistance gene in Brassica oleracea // Mol. Breed. 2012. № 30. P. 809–818. https://doi.org/10.1007/s11032-011-9665-8
Bosland P.W., Williams P.H., Morrison R.H. Influence of soil temperature on the expression of yellows and wilt of crucifers by Fusarium oxysporum // Plant Dis. 1988. № 72. P. 777–780. https://doi.org/10.1094/PD-72-0777
Farnham M.W., Keinath A.P., Smith J.P. Characterization of fusarium yellows resistance in collard // Plant Dis. 2001. № 85. P. 890–894. https://doi.org/10.1094/PDIS.2001.85.8.890
Berrocal-Lobo M., Molina A. Arabidopsis defense response against Fusarium oxysporum // Trends Plant Sci. 2007. № 13. P. 145–150. https://doi.org/10.1016/j.tplants.2007.12.004
Королева С.В., Дякунчак С.А., Ситников С.В. Иммунологическая оценка селекционного материала при создании гибридов F1 белокочанной капусты с групповой устойчивостью к фузариозу и сосудистому бактериозу (методические рекомендации). М.: 2012. 16 с.
Arden S. Fusarium Yellows of Cabbage and Related Crops. N.Y.: Vegetable Crops, 1979. 730 p.
Keinath A.P., Farnham M.W., Smith P. Reactions of 26 cultivars of Brassica oleracea to yellows in naturally infested soil // Biol. Cult. Tests. 1998. № 13. 155 p.
Collard B.C.Y., Jahufer M.Z.Z., Brouwer J.B., Pang E.C.K. An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts // Euphytica. 2005. № 142. P. 169–196. https://doi.org/10.1007/s10681-005-1681-5
Vali U., Brandstrom M., Johansson M., Ellegren H. Insertion-deletion polymorphisms (indels) as genetic markers in natural populations // BMC Genetic. 2008. № 9. https://doi.org/10.1186/1471-2156-9-8
Lv H. Fang Z., Yang L. et al. Research on screening of resistant resources to fusarium wilt and inheritance of the resistant gene in cabbage // Acta Horticult. Sinica. 2011. V. 5. № 38. P. 875–885. https://doi.org/10.16420/j.issn.0513-353x.2011.05.001
Дубина Е.B. ДНК-технологии (молекулярное маркирование) в селекции риса и семеноводстве овощных культур: Дис. … д-ра биοл. наук. Краснодар: ВНИИ риса, 2019. 275 с.
Фесенко И.А., Куклев М.Ю., Карлов Г.И. Создание ДНК-маркера устойчивости томата к фузариозному увяданию // Известия ТСХА. № 1. 2007. С. 66–72.
Lv H., Yang L., Kang J. et al. Development of InDel markers linked to fusarium wilt resistance in cabbage // Mol. Breed. 2013. V. 32. P. 961–967. https://doi.org/10.1007/s11032-013-9925-x
Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA // Nucl. Ac. Res. 1980. V. 10. P. 4321–4325.
Liu X., Han F., Kong C. Rapid introgression of the fusarium wilt resistance gene into an elitecabbage line through the combined application of a microspore culture, genome background analysis, and disease resistance-specific marker assisted foreground selection // Front. in Plant Sci. 2017. V. 8. Article 11. https://doi.org/10.3389/fpls.2017.00354
Кутлунина Н.А., Ермошин А.А. Молекулярно-генетические методы в исследовании растений. Екатеринбург, 2017. 142 с.
Батин Н.В. Компьютерный статистический анализ данных. Минск, 2008. 160 с.
Нгуен М.Л., Монахос Г.Ф., Комахин Р.А., Монахос С.Г. Новый локус устойчивости к киле в хромосоме А05 капусты пекинской (Brassica rapa L.) // Генетика. 2018. Т. 54. № 3. С. 306–315.
Аджиева В.Ф., Некрашевич Н.А., Малышев С.В. и др. Сравнительный анализ полиморфизма микросателлитных маркеров генотипов томата (Solanum lycopersicum L.) белорусской и зарубежной селекции // Мол. и прикладная генетика. 2010. Т. 11. С. 7–11.
Ramirez-Villupadua J., Endo R.M., Bosland P., Wiliams P.H. A new race of Fusarium oxysporum f. sp. conglutinans that attacks cabbage with type A resistance // Plant Dis. 1985. № 69. P. 612–613.