Research Article
Merinos Koyunların Kan, Seminal Plazma Testosteron, Büyüme Hormonu ve Kortizol Düzeylerinin Sperm Kalitesi Üzerine Etkisi
Abstract
Testosteron, büyüme hormonu ve kortizol düzeylerinin sperm kalitesine etkisini belirlemek için 10 adet Merinos koç üzerinde araştırma yapıldı. Testosteronun kan ve seminal plazma seviyeleri arasında anlamlı bir ilişki tespit edildi (P ˂ 0.01). Seminal plazmada ölçülebilir düzeyde GH saptanmamış, plazma GH düzeylerinin, tıpkı testosteron düzeyleri gibi üreme mevsiminin yaklaşmasıyla birlikte artmaya başladığı ve sezon başında pik yaptığı saptanmıştır. Plazma ve seminal plazma kortizol seviyeleri ise sezon öncesinde yükselirken sezon başında önemli ölçüde azalmış ve plazma ve seminal plazma seviyeleri arasında dikkate değer bir korelasyon tespit edilmiştir (P ˂ 0.01). Merinos koçlarında plazma GH seviyeleri ile seminal plazma kortizol seviyeleri arasında gözlenen önemli bir negatif ilişki. İncelenen hormonların sperm kalitesi üzerinde anlamlı bir etkisinin olmadığı ancak sadece plazma GH seviyeleri ile Merinostaki sperm hacimleri arasında önemli bir negatif ilişki olduğu tespit edildi (P ˂ 0.01). Sonuç olarak koçlarda özellikle plazma testosteron ve kortizol düzeylerinin plazma düzeylerini belirlediği; hem plazma hem de seminal plazma testosteron seviyeleri üreme mevsiminde önemli ölçüde artar.
Keywords
Supporting Institution
Selçuk üniversitesi BAP koordinatörlüğü
Project Number
09401085
References
- Abecia, J.A., Forcada, F., Gonzalez-Bulnes, A., 2012. Hormonal control of reproduction in small ruminants. Anim. Reprod. Sci. 130, 173-179.
- Akalın, P.P., Başpınar, N., Çoyan, K., Bucak, M.N., Ataman, M.B., Ömür, A., Bi̇lgi̇li̇, A., Güngör, Ş., Sarıözkan, S., Öztürk, C., Bodu, M., Aci̇baeva, B., 2015. Metiyonin ve trehaloz içeren sulandırıcıların koç spermasının dondurma-çözdürme sonrası sperm akrozom bütünlüğü ve biyokimyasal parametreleri üzerine etkileri. Bahri Dağdas Hay. Ars. Derg., 3, 7-15.
- Alvarez, M., Tamayo- Canul, J., Anel, E., Boixo, J.C., Mata-Campuzano, M., Martinez-Pastor, F., Anel, L., Paz., P., 2012. Sperm concentration at freezing affects post-thaw quality and fertility on ram semen. Theriogenology, 77, 1111-1118.
- Ansari, S.P., Singh, H.S., Patel, P., Mishra, A., 2017. Biochemical and hormonal profile of epididymis of buck during winter and summer season. Indian J. Anim. Res. 51, 449-452.
- Bartlett, J.M., Charlton, H.M., Robinson, I.C., Nieschlag, E., 1990. Pubertal development and testicular function in the male growth hormone-deficient rat. J. Endocrinol, 126, 193-201.
- Belkadi S, Safsaf B, Heleili N, Tlidjane, M., Belkacem, L., Oucheriahet, Y., 2017. Seasonal influence on sperm parameters, scrotal measurements, and serum testosterone in ouled djellal breed rams in Algeria. Vet. World. 10, 1486-1492.
- Benmoula, A., Badi, A., El Fadili, M., EL Khalil, K., Allai, L., 2017. Effect of season on scrotal circumference, semen characteristics, seminal plasma composition and spermatozoa motility during liquid storage in INRA180 rams. Anim. Reprod. Sci., 180, 17-22.
- Bex, F., Bartke, A., Goldman, B.D., Dalterio, S., 1978. Prolactin, growth hormone, luteinizing hormone receptors, and seasonal changes in testicular activity in the golden hamster. Endocrinology, 103, 2069-2080.
- Boland, M.P., Al Kamari, A.A., Crosby, T.F., Haynes, N.B., Howles, Kelleher D.L., Gordonet, I., 1985. The influence of breed, season and photoperiod on semen characteristics, testicular size, libido and plasma hormone concentrations in rams. Anim. Reprod. Sci., 9, 241-252.
- Câmara, D.R., Mello-Pinto, M.M.C., Pinto, L.C., Brasil, O.O., Nunes, J.F., Guerra, M.M.P., 2011. Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen. Small Rumin. Res., 100, 44-49.
- Casao, A., Perez, R., Abecia, A., Forcada, F., Muiño-Blanco, T., Cebrián-Pérez, J. Á., 2013. The effect of exogenous melatonin during the non-reproductive season on the seminal plasma hormonal profile and the antioxidant defence system of Rasa Aragonesa rams. Anim. Reprod. Sci., 138, 168-174.
- David, I., Kohnke, P., Lagriffoul, G., Praud, O., Plouarboue, F., 2015. Mass sperm motility is associated with fertility in sheep. Anim. Reprod. Sci., 161, 75-81.
- Graves, C.N., Elier, H., 1979. Cortisol content of semen and the effect of exogenous cortisol on the concentration of cortisol and minerals (Ca, Mg, K and Na) in semen and blood plasma of bulls. Biol. Reprod. 21, 1225-1229.
- Hamidi, A., Mamomei, M., Khalil, M., Tabatabaei, S., Hedayat-allah, R., 2012. Correlation between blood growth hormone profile and reproduction performance in Arabic rams. Comp. Clin. Path. 21, 819-823.
- İleri, İ.K., Ak, K., Pabuççuoğlu, S., Birler, S., 1998. Evcil hayvanlarda reprodüksiyon ve suni tohumlama. İÜ Vet Fak Yayınları, İstanbul Türkiye.
- Javed, M.T., Khan, A., Ali, M., 2000. Influence of season on seminal plasma testosterone and oestrogen in healthy and abnormal buffalo bulls and their relationship with other semen parameters. Vet. Arh., 70, 141-149.
- Kalra, S., Kalra, B., Sharma, A., 2008. Growth hormone improves semen volume, sperm count and motility in men with idiopathic normogonadotropic infertility. Endocrine Abstracts, 16, 613.
- King, B.J., Da Silva, V.V., Harper, J.D., Scott, C.J., Sillence, M.N., 2005. Equine growth hormone enhances motility and extends longevity of ram spermatozoa in vitro. Reprod. Fertil. Dev., 17, 85.
- Maurya, V.P., Seljan, V., Kumar, D., Naqvi, S.M.K., 2010. Effect of induced body condition score differences on sexual behavior, scrotal measurements, semen attributes and endocrine responses in Malpura rams under hot semi‐arid environment. J. Anim. Physiol. Anim. Nutr., 94, 308-317.
- Mickelsen, D.W., Paisley, L.G., Dahmen, J.J., 1981. The effects of season on the scrotal circumference and sperm motility and morphology in rams. Theriogenology, 16, 45-51.
- Moore, R.W., 1985. A comparison of electro-ejaculation with the artifical vagina for ram semen collection. N. Z. Vet. J., 33, 22-23.
- Ömür, A.D., Çoyan, K., 2014. Sezon içi ve sezon dışında koç spermasının dondurulmasında antioksidanların etkisi. Erciyes Üniv. Vet. Fak. Derg., 11, 37-42.
- Palacios, C., Alvarez, S., Martín-Gil, J., Abecia, A., 2016. Plasma hormonal levels of rams are affected by sexual activity and confinement in a semen collection centre. ARC J. Anim. Vet. Sci. 2, 15-21.
The Influence of blood, seminal plasma testosterone, growth hormone and cortisol levels on the sperm quality in merino rams
Abstract
The research was conducted on 10 Merino rams to determine the effect of testosterone, growth hormone and cortisol levels on the sperm quality. A significant relationship was detected between blood and seminal plasma levels of testosterone (P ˂ 0.01). No GH on a measurable level was detected in the seminal plasma, and it was determined that plasma GH levels, just as testosterone levels began to increase with the approach of the reproductive season, and peaked at the start of the season. Plasma and seminal plasma cortisol levels, on the other hand, while increasing before the season, decreased significantly at the start of the season, and a remarkable correlation between plasma and seminal plasma levels was ascertained (P ˂ 0.01). An important negative relation which was observed between plasma GH levels and seminal plasma cortisol levels in Merino rams. It was found out that the hormones that were examined did not have a significant influence on sperm qualities, but there was an important negative relation only between the plasma GH levels and sperm volumes in the Merino (P ˂ 0.01). In conclusion, it may be stated that in rams, especially plasma testosterone and cortisol levels determine the plasma levels; both plasma and seminal plasma testosterone levels increase to a significant degree in the reproductive season.
Keywords
Project Number
09401085
References
- Abecia, J.A., Forcada, F., Gonzalez-Bulnes, A., 2012. Hormonal control of reproduction in small ruminants. Anim. Reprod. Sci. 130, 173-179.
- Akalın, P.P., Başpınar, N., Çoyan, K., Bucak, M.N., Ataman, M.B., Ömür, A., Bi̇lgi̇li̇, A., Güngör, Ş., Sarıözkan, S., Öztürk, C., Bodu, M., Aci̇baeva, B., 2015. Metiyonin ve trehaloz içeren sulandırıcıların koç spermasının dondurma-çözdürme sonrası sperm akrozom bütünlüğü ve biyokimyasal parametreleri üzerine etkileri. Bahri Dağdas Hay. Ars. Derg., 3, 7-15.
- Alvarez, M., Tamayo- Canul, J., Anel, E., Boixo, J.C., Mata-Campuzano, M., Martinez-Pastor, F., Anel, L., Paz., P., 2012. Sperm concentration at freezing affects post-thaw quality and fertility on ram semen. Theriogenology, 77, 1111-1118.
- Ansari, S.P., Singh, H.S., Patel, P., Mishra, A., 2017. Biochemical and hormonal profile of epididymis of buck during winter and summer season. Indian J. Anim. Res. 51, 449-452.
- Bartlett, J.M., Charlton, H.M., Robinson, I.C., Nieschlag, E., 1990. Pubertal development and testicular function in the male growth hormone-deficient rat. J. Endocrinol, 126, 193-201.
- Belkadi S, Safsaf B, Heleili N, Tlidjane, M., Belkacem, L., Oucheriahet, Y., 2017. Seasonal influence on sperm parameters, scrotal measurements, and serum testosterone in ouled djellal breed rams in Algeria. Vet. World. 10, 1486-1492.
- Benmoula, A., Badi, A., El Fadili, M., EL Khalil, K., Allai, L., 2017. Effect of season on scrotal circumference, semen characteristics, seminal plasma composition and spermatozoa motility during liquid storage in INRA180 rams. Anim. Reprod. Sci., 180, 17-22.
- Bex, F., Bartke, A., Goldman, B.D., Dalterio, S., 1978. Prolactin, growth hormone, luteinizing hormone receptors, and seasonal changes in testicular activity in the golden hamster. Endocrinology, 103, 2069-2080.
- Boland, M.P., Al Kamari, A.A., Crosby, T.F., Haynes, N.B., Howles, Kelleher D.L., Gordonet, I., 1985. The influence of breed, season and photoperiod on semen characteristics, testicular size, libido and plasma hormone concentrations in rams. Anim. Reprod. Sci., 9, 241-252.
- Câmara, D.R., Mello-Pinto, M.M.C., Pinto, L.C., Brasil, O.O., Nunes, J.F., Guerra, M.M.P., 2011. Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen. Small Rumin. Res., 100, 44-49.
- Casao, A., Perez, R., Abecia, A., Forcada, F., Muiño-Blanco, T., Cebrián-Pérez, J. Á., 2013. The effect of exogenous melatonin during the non-reproductive season on the seminal plasma hormonal profile and the antioxidant defence system of Rasa Aragonesa rams. Anim. Reprod. Sci., 138, 168-174.
- David, I., Kohnke, P., Lagriffoul, G., Praud, O., Plouarboue, F., 2015. Mass sperm motility is associated with fertility in sheep. Anim. Reprod. Sci., 161, 75-81.
- Graves, C.N., Elier, H., 1979. Cortisol content of semen and the effect of exogenous cortisol on the concentration of cortisol and minerals (Ca, Mg, K and Na) in semen and blood plasma of bulls. Biol. Reprod. 21, 1225-1229.
- Hamidi, A., Mamomei, M., Khalil, M., Tabatabaei, S., Hedayat-allah, R., 2012. Correlation between blood growth hormone profile and reproduction performance in Arabic rams. Comp. Clin. Path. 21, 819-823.
- İleri, İ.K., Ak, K., Pabuççuoğlu, S., Birler, S., 1998. Evcil hayvanlarda reprodüksiyon ve suni tohumlama. İÜ Vet Fak Yayınları, İstanbul Türkiye.
- Javed, M.T., Khan, A., Ali, M., 2000. Influence of season on seminal plasma testosterone and oestrogen in healthy and abnormal buffalo bulls and their relationship with other semen parameters. Vet. Arh., 70, 141-149.
- Kalra, S., Kalra, B., Sharma, A., 2008. Growth hormone improves semen volume, sperm count and motility in men with idiopathic normogonadotropic infertility. Endocrine Abstracts, 16, 613.
- King, B.J., Da Silva, V.V., Harper, J.D., Scott, C.J., Sillence, M.N., 2005. Equine growth hormone enhances motility and extends longevity of ram spermatozoa in vitro. Reprod. Fertil. Dev., 17, 85.
- Maurya, V.P., Seljan, V., Kumar, D., Naqvi, S.M.K., 2010. Effect of induced body condition score differences on sexual behavior, scrotal measurements, semen attributes and endocrine responses in Malpura rams under hot semi‐arid environment. J. Anim. Physiol. Anim. Nutr., 94, 308-317.
- Mickelsen, D.W., Paisley, L.G., Dahmen, J.J., 1981. The effects of season on the scrotal circumference and sperm motility and morphology in rams. Theriogenology, 16, 45-51.
- Moore, R.W., 1985. A comparison of electro-ejaculation with the artifical vagina for ram semen collection. N. Z. Vet. J., 33, 22-23.
- Ömür, A.D., Çoyan, K., 2014. Sezon içi ve sezon dışında koç spermasının dondurulmasında antioksidanların etkisi. Erciyes Üniv. Vet. Fak. Derg., 11, 37-42.
- Palacios, C., Alvarez, S., Martín-Gil, J., Abecia, A., 2016. Plasma hormonal levels of rams are affected by sexual activity and confinement in a semen collection centre. ARC J. Anim. Vet. Sci. 2, 15-21.
There are 23 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Articles |
| Authors | |
| Project Number | 09401085 |
| Publication Date | December 31, 2022 |
| Submission Date | October 28, 2022 |
| Acceptance Date | December 29, 2022 |
| Published in Issue | Year 2022 Volume: 7 Issue: 3 |
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