Susceptibility of Japanese Medaka to Edwardsiella piscicida and E. anguillarum Isolated from Aquacultured Fish

Aki Nishihara; Takechiyo Sumiyoshi; Koushiro Arakawa; Natsuki Morimoto; Shinya Yasumoto; Masakazu Kondo; Tomoya Kono; Masahiro Sakai; Jun-ichi Hikima

doi:10.3147/jsfp.58.175

Short Communications

Susceptibility of Japanese Medaka to Edwardsiella piscicida and E. anguillarum Isolated from Aquacultured Fish

Aki Nishihara, Takechiyo Sumiyoshi, Koushiro Arakawa, Natsuki Morimoto, Shinya Yasumoto, Masakazu Kondo, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima

Author information

Aki Nishihara
Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
Takechiyo Sumiyoshi
Graduate School of Agriculture, University of Miyazaki
Koushiro Arakawa
Faculty of Agriculture, University of Miyazaki
Natsuki Morimoto
Faculty of Agriculture, University of Miyazaki
Research Fellowship for Young Scientists, Pathology Division, Nansei Main Station, Aquaculture Research Department, Fisheries Technology Institute, Japan Fisheries Research and Education Agency
Shinya Yasumoto
Department of Applied Aquabiology, National Fisheries University, Japan Fisheries Research and Education Agency
Masakazu Kondo
Department of Applied Aquabiology, National Fisheries University, Japan Fisheries Research and Education Agency
Tomoya Kono
Graduate School of Agriculture, University of Miyazaki
Faculty of Agriculture, University of Miyazaki
Masahiro Sakai
Graduate School of Agriculture, University of Miyazaki
Faculty of Agriculture, University of Miyazaki
Jun-ichi Hikima
Graduate School of Agriculture, University of Miyazaki
Faculty of Agriculture, University of Miyazaki

Keywords: Edwardsiellosis, Edwardsiella piscicida , Edwardsiella anguillarum , Japanese medaka Oryzias latipes, Immersion infection model

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2023 Volume 58 Issue 4 Pages 175-179

DOI https://doi.org/10.3147/jsfp.58.175

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Abstract

Edwardsiella bacteria infect Japanese medaka Oryzias latipes; however, the difference in susceptibility between E. piscicida and E. anguillarum is unknown. In this study, we performed challenge tests with medaka using four Edwardsiella strains, which were difficult to be identified by specific primers. The four strains were reclassified as three E. piscicida strains and one E. anguillarum strain using phylogenetic analysis. The immersion challenge demonstrated that medaka were more susceptible to E. piscicida than to E. anguillarum. Increases in the bacterial copy number in the infected medaka kidneys were correlated with the worsening of the external symptoms. Medaka is considered a valuable model organism for understanding the etiology of edwardsiellosis.

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