It is necessary to solve the problem of a level difference on floors from the viewpoint where it walks safely and comfortably in buildings. The one step stair and the slope are general methods of solving a level difference greatly not large. In this research, physiological loads in the one step stairs and the slopes were compared. The results are as follows. ・The one step stairs and the slopes to give an equivalent physiological load, by the same height difference, exist in the range of level difference of 25cm or less. ・When it is examined that the height of the one step stairs and the slopes length, physically handicapped passengers abilities should be assumed to be the main index.