Background: Neointimal hyperplasia (NIH) is the pathological basis of
vascular injury disease. Vascular cells are the dominant cells in the process of
NIH, but the extent of heterogeneity amongst them is still unclear.
Methods: A mouse model of NIH was constructed by inducing carotid artery
ligation. Single-cell sequencing was then used to analyze the transcriptional
profile of vascular cells. Cluster features were determined by functional
enrichment analysis, gene set scoring, pseudo-time analysis, and cell-cell
communication analysis. Additionally, immunofluorescence staining was conducted
on vascular tissues from fibroblast lineage-traced (Pdgfra
Announcements
Open Access
Original Research
Single-Cell Transcriptome Analysis Reveals Dynamic Populations of Vascular Cells in Neointimal Hyperplasia
Guangzheng Shi1, Xinran Tong1, Weihong Sun2, Zilong Fang1, Wendong Chen1, Gonghao Jiang1, Peili Zhang1, Qun Li1,*
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1
The Department of Cardiovascular Medicine, State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Hypertension, Ruijin Hospital, Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine, 200025 Shanghai, China
2
Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences (CAS), 200031 Shanghai, China
*Correspondence: liqun@sibs.ac.cn (Qun Li)
Front. Biosci. (Landmark Ed) 2024, 29(5), 173;
https://doi.org/10.31083/j.fbl2905173
Submitted: 29 December 2023 | Revised: 18 February 2024 | Accepted: 13 March 2024 | Published: 6 May 2024
Copyright: © 2024 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract
Keywords
neointimal hyperplasia
fibroblasts
endothelial cells
vascular smooth muscle cells
single-cell RNA sequencing
Funding
82070509/National Natural Science Foundation of China
SHSMU-ZDCX20212700/Innovative research team of high-level local universities in Shanghai
Figures
Fig. 1.