Pathotogenicity of Turkish Entomopathogenic nematodes, Steinernema feltiae and Steinernema carpocapsae (Rhabditida: Steinernematidae) on the rice weevil: Sitophilus oryzae (Coleoptera: Curculionidae)
Öz
Stored product pests are one of the most important biotic factors that cause serious postharvest losses of food crops in the course of storage. To control these pests, pesticides have been extensively used for many years. However, it is known that pesticides and their residues have toxic effects on non-target organisms. Therefore, it is believed that Entomopathogenic Nematodes (EPNs), which have been widely employed for biological control in agricultural fields, can be used for the potential control of Sitophilus oryzae L., 1763 (Coleoptera: Curculionidae). In this study, the biocontrol potential of Steinernema feltiae TUR-S3 and Steinernema carpocapsae TUR-S4 isolates (Rhabditida: Steinernema) were evaluated against the adults of S. oryzae at eight different concentrations [250 IJs/cm2 (600 IJs/adult), 200 IJs/cm2 (480 IJs/adult), 100 IJs/cm2 (240 IJs/adult), 50 IJs/cm2 (120 IJs/adult), 25 IJs/cm2 (60 IJs/adult), 12 IJs/cm2 (30 IJs/adult), 6 IJs/cm2 (15 IJs/adult), and 3 IJs/cm2 (7.5 IJs/adult)]. According to the results, both S. feltiae TUR-S3 and S. carpocapsae TUR-S4 exhibited the highest mortality rate (96.67%) on S. oryzae when applied at the concentration of 250 IJs/cm2. The most effective LC50 and LC90 values for S. feltiae TUR-S3 were determined as 47.55 and 167.16, respectively. These values were obtained as 35.66 and 121.79 for S. carpocapsae TUR-S4 isolate.
Anahtar Kelimeler
Steinernema carpocapsae Steinernema feltiae Sitophilus oryzae rice weevil biological control
Destekleyen Kurum
TÜBİTAK
Proje Numarası
219O370
Teşekkür
This study was financially supported by the TUBITAK (The Scientific and Technological Research Council of Türkiye), Project number: 219O370. Prof. Dr. Özgür SAĞLAM is thanked for providing the rice weevil. Additionally, Dr. Tufan Can ULU is thanked for providing statistical support.
Kaynakça
- Ali, I. and Jain, C.K. 1998. Groundwater contamination and health hazards by some of the most commonly used pesticides. Current Science, 75 (10): 1011-1014.
- Burnell, A. and Stock, S.P. 2000. Heterorhabditis, Steinernema and their bacterial symbionts—lethal pathogens of insects. Nematology, 2 (1): 31-42.
- Canhilal, R., and Yüksel, 2020. E. Laboratory evaluation of some native isolates of Steinernema feltiae against the Rice Weevil, Sitophilus oryzae (L.), (Curculionidae: Coleoptera). Türkiye Biyolojik Mücadele Dergisi, 11(1): 65-69.
- Dede, E., Bütüner, A.K. and Susurluk, İ.A. 2022. Biocontrol potential of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain against the beet webworm, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae). Turkish Journal of Entomology, 46 (4): 399-405
- Dubey, N.K., Srivastava, B. and Kumar, A. 2008. Current status of plant products as botanical pesticides in storage pest management. Journal of biopesticides, 1 (2): 182-186.
- Ehlers, R.U., Stoessel, S. and Wyss, U. 1990. The influence of phase variants of Xenorhabdus spp. and Escherichia coli (Enterobacteriaceae) on the propagation of entomopathogenic nematodes of the genera Steinernema and Heterorhabditis. Revue de nématologie, 13 (4): 417-424.
- Flexner, J., Lighthart, B. and Croft, B.A. 1986. The effects of microbial pesticides on non-target, beneficial arthropods. Agriculture, ecosystems & environment, 16 (3-4): 203-254.
- Gaugler, R. 2002. Entomopathogenic Nematology. CABI Publishing, Wallingford, UK, 394 pp.
- Gaugler, R., Lewis, E. and Stuart, R.J. 1997. Ecology in the service of biological control: the case of entomopathogenic nematodes. Oecologia, 109: 483-489.
- Govindan, K. and Nelson, S.J. 2009. Insecticidal activity of twenty plant powders on mortality, adult emergence of Sitophilus oryzae L. and grain weight loss in paddy. Journal of Biopesticides, 2 (2): 169-172.
- Grewal, P.S., Ehlers, R.U. and Shapiro-Ilan, D.I. 2005. Critical issues and research needs for expanding the use of nematodes in biological control. In: Nematodes as biocontrol agents. Grewal, P.S., Ehlers, R.U. and Shapiro-Ilan, D.I. (eds.), CABI, Wallingford, UK, (pp. 479-489).
- Hamel, D., Rozman, V. and Liška, A. 2020. Storage of Cereals in Warehouses with or without Pesticides. Insects, 11 (12): 846.
- Javed, S., Khanum, T.A. and Khan, S. 2020. Biocontrol potential of entomopathogenic nematode species against Tribolium confusum (Jac.)(Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Fab.)(Coleoptera: Bostrichidae) under laboratory conditions. Egyptian Journal of Biological Pest Control, 30: 1-6.
- Karan, S., Agrawal, N.S. and Girish, G.K. 1974. Studies on the quantitative loss in various high yielding varieties of maize, due to Sitophilus oryzae (L.) Col., Curculionidae. Labdev Journal of Science and Technology, 12 (1): 3-4.
- Kavallieratos, N.G., Athanassiou, C.G., Saitanis, C.J., Kontodimas, D.C., Roussos, A.N., Tsoutsa, M.S. and Anastassopoulou, U.A. 2007. Effect of two azadirachtin formulations against adults of Sitophilus oryzae and Tribolium confusum on different grain commodities. Journal of food protection, 70 (7): 1627-1632.
- Kunkel, B.A., Shapiro-Ilan, D.I., Campbell, J.F. and Lewis, E.E. 2006. Effect of Steinernema glaseri-infected host exudates on movement of conspecific infective juveniles. Journal of Invertebrate Pathology, 93 (1): 42-49.
- Laznik, Ž., Tóth, T., Lakatos, T., Vidrih, M. and Trdan, S. 2010. The activity of three new strains of Steinernema feltiae against adults of Sitophilus oryzae under laboratory conditions. Journal of Food, Agriculture and Environment, 8 (1): 150-154.
- Lunau, S., Stoessel, S., Schmidt-Peisker, A.J. and Ehlers, R.U. 1993. Establishment of monoxenic inocula for scaling up in vitro cultures of the entomopathogenic nematodes Steinernema spp. and Heterorhabditis spp. Nematologica, 39 (1-4): 385-399.
- Manosathiyadevan, M., Bhuvaneshwari, V. and Latha, R. 2017. Impact of insects and pests in loss of crop production: a review. Sustainable agriculture towards food security, 2017: 57-67.
- Muda, A.R. 1986. Pest problems and the use of pesticides in grain storage in Malaysia. ACIAR Proceedings Series, Australian Centre for International Agricultural Research, 1986: 11-16.
- Neethirajan, S., Karunakaran, C., Jayas, D.S. and White, N.D.G. 2007. Detection techniques for stored-product insects in grain. Food Control 18: 157-162.
- Negrisoli, C.R.D.C.B., Júnior, A.S.N., Bernardi, D. and Garcia, M.S. 2013. Activity of eight strains of entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) against five stored product pests. Experimental parasitology, 134 (3): 384-388.
- Oerke, E.C. and Dehne, H.W. 2004. Safeguarding production—losses in major crops and the role of crop protection. Crop protection, 23 (4): 275-285.
- Pereira, J.L., Antunes, S.C., Castro, B.B., Marques, C.R., Gonçalves, A.M., Gonçalves, F. and Pereira, R. 2009. Toxicity evaluation of three pesticides on non-target aquatic and soil organisms: commercial formulation versus active ingredient. Ecotoxicology, 18: 455-463.
- Rumbos, C.I. and Athanassiou, C.G. 2017. The use of entomopathogenic nematodes in the control of stored-product insects. Journal of Pest Science, 90: 39-49.
- Schöller, M., Flinn, P.W., Grieshop, M.J. and Zdarkova, E. 2006. Biological control of stored product pests. Insect management for food storage and processing, 2006: 67-87.
- Shapiro-Ilan, D.I., Gouge, D.H., Piggott, S.J. and Fife, J.P. 2006. Application technology and environmental considerations for use of entomopathogenic nematodes in biological control. Biological control, 38 (1): 124-133.
- Strauch, O. and Ehlers, R.U. 1998. Food signal production of Photorhabdus luminescens inducing the recovery of entomopathogenic nematodes Heterorhabditis spp. in liquid culture. Applied microbiology and biotechnology, 50: 369-374.
- Sunanda, B.S., Siddiqui, A.U. and Sharma, S. 2012. Effect of temperature on longevity of entomopathogenic nematodes, Steinernema abbasi and Heterorhabditis indica. Indian journal of nematology, 42 (1): 17-19.
- Susurluk, A. and Ehlers, R.U. 2008. Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. BioControl, 53: 627-641.
- Susurluk, A., Şahin, Y.S. and Bouhari, A. 2018. Effects of some inorganic fertilizers on the entomopathogenic nematodes Steinernema feltiae (Tur-S3) and Heterorhabditis bacteriophora (HBH). Türkiye Biyolojik Mücadele Dergisi, 9 (2): 73-81.
- Swamy, K.N., Mutthuraju, G.P., Jagadeesh, E. and Thirumalaraju, G.T. 2014. Biology of Sitophilus oryzae (L.) (Coleoptera: Curculionidae) on stored maize grains. Current Biotica, 8 (1): 76-81.
- Şahin, Y.S., Boucharı, A., Ulu, T.C., Sadıç, B. and Susurluk, A. 2018. New application method for entomopathogenic nematode Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae) HBH strain against Locusta migratoria (Linnaeus, 1758) (Orthoptera: Acrididae). Turkish Journal of Entomology, 42 (4): 305-312.
- Trdan, S., Vidrih, M. and Valič, N. 2006. Activity of four entomopathogenic nematode species against young adults of Sitophilus granarius (Coleoptera: Curculionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae) under laboratory conditions/Wirkung von vier entomopathogenen Nematodenarten gegenüber Sitophilus granarius (Coleoptera: Curculionidae) und Oryzaephilus surinamensis (Coleoptera: Silvanidae) unter Laborbedingungen. Journal of plant diseases and protection, 2006: 168-173.
- Ulu, T.C., Sadic, B., Susurluk, I.A. and Aksit, T. 2015. Virulence of four entomopathogenic nematode species for plum sawfly, Hoplocampa flava L. (Hymenoptera: Tenthredinidae). Invertebrate Survival Journal, 12 (1): 274-277.
- Wouts, W.M. 1990. The primary form of Xenorhabdus species (Enterobacteriaceae: Eubacteriales) may consist of more than one bacterial species. Nematologica, 36 (1-4): 313-318.
- Zaller, J.G. and Brühl, C.A. 2019. Non-target effects of pesticides on organisms inhabiting agroecosystems. Frontiers in Environmental Science, 7: 75. doi: 10.3389/fenvs.2019.00075.
- Yüksel, E., Canhilal, R. and Imren, M. 2019. Potential of four Turkish isolates of entomopathogenic nematodes against three major stored products insect pests. Journal of Stored Products Research, 83: 317-321..
Türk Entomopatojen Nematodların, Steinernema feltiae ve Steinernema carpocapsae (Rhabditida: Steinernematidae) Pirinç Biti Sitophilus oryzae (Coleoptera: Curculionidae) Üzerinde Patojenisitesi
Öz
Depolanmış ürün zararlıları, tarım ürünlerin depolama sürecinde hasat sonrası ciddi kayıplara neden olan en önemli biyotik faktörlerden biridir. Bu zararlılar ile mücadele amacıyla yıllardır yaygınlıkla pestisitler kullanılmaktadır. Ancak pestisitlerin ve ne olduğu kalıntıların hedef dışı organizmalar üzerinde oluşturduğu toksik etkiler bilinmektedir. Bu nedenle, tarım alanlarında biyolojik mücadelede yaygın olarak kullanılan Entomopatojen Nematodların (EPN), Sitophilus oryzae L., 1763 (Coleoptera: Curculionidae)'nin potansiyel kontrolünde kullanılabileceği düşünülmektedir. Bu çalışmada Steinernema feltiae TUR-S3 ve Steinernema carpocapsae TUR-S4 izolatlarının (Rhabditida: Steinernema) S. oryzae' nin erginleri üzerindeki biyokontrol potansiyel 8 farklı konsantrasyonda [250 IJs/cm2 (600 IJs/ergin), 200 IJs/cm2 (480 IJs/ergin), 100 IJs/cm2 (240 IJs/ergin), 50 IJs/cm2 (120 IJs/ergin), 25 IJs/cm2 (60 IJs/ergin), 12 IJs/cm2 (30 IJs/ergin), 6 IJs/cm2 (15 IJs/ergin), ve 3 IJs/cm2 (7.5 IJs/ergin)] değerlendirilmiştir. Sonuçlara göre hem S. feltiae TUR-S3 izolatı hemde S. carpocapsae TUR-S4 izolatı, S. oryzae erginlerinde en yüksek ölüm oranını (96.67%) 250 IJs/cm2 uygulama konsantrasyonunda gerçekleştirmiştir. S. feltiae TUR-S3 için en etkili LC50 ve LC90 değerleri sırasıyla 47.55 ve 167.16 dir. Bu değerler S. carpocapsae TUR-S4 izolatı için 35.66 ve 121.79 olarak elde edilmiştir.
Anahtar Kelimeler
Steinernema carpocapsae Steinernema feltiae Sitophilus oryzae pirinç biti biyolojik mücadele
Proje Numarası
219O370
Kaynakça
- Ali, I. and Jain, C.K. 1998. Groundwater contamination and health hazards by some of the most commonly used pesticides. Current Science, 75 (10): 1011-1014.
- Burnell, A. and Stock, S.P. 2000. Heterorhabditis, Steinernema and their bacterial symbionts—lethal pathogens of insects. Nematology, 2 (1): 31-42.
- Canhilal, R., and Yüksel, 2020. E. Laboratory evaluation of some native isolates of Steinernema feltiae against the Rice Weevil, Sitophilus oryzae (L.), (Curculionidae: Coleoptera). Türkiye Biyolojik Mücadele Dergisi, 11(1): 65-69.
- Dede, E., Bütüner, A.K. and Susurluk, İ.A. 2022. Biocontrol potential of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain against the beet webworm, Loxostege sticticalis L., 1761 (Lepidoptera: Pyralidae). Turkish Journal of Entomology, 46 (4): 399-405
- Dubey, N.K., Srivastava, B. and Kumar, A. 2008. Current status of plant products as botanical pesticides in storage pest management. Journal of biopesticides, 1 (2): 182-186.
- Ehlers, R.U., Stoessel, S. and Wyss, U. 1990. The influence of phase variants of Xenorhabdus spp. and Escherichia coli (Enterobacteriaceae) on the propagation of entomopathogenic nematodes of the genera Steinernema and Heterorhabditis. Revue de nématologie, 13 (4): 417-424.
- Flexner, J., Lighthart, B. and Croft, B.A. 1986. The effects of microbial pesticides on non-target, beneficial arthropods. Agriculture, ecosystems & environment, 16 (3-4): 203-254.
- Gaugler, R. 2002. Entomopathogenic Nematology. CABI Publishing, Wallingford, UK, 394 pp.
- Gaugler, R., Lewis, E. and Stuart, R.J. 1997. Ecology in the service of biological control: the case of entomopathogenic nematodes. Oecologia, 109: 483-489.
- Govindan, K. and Nelson, S.J. 2009. Insecticidal activity of twenty plant powders on mortality, adult emergence of Sitophilus oryzae L. and grain weight loss in paddy. Journal of Biopesticides, 2 (2): 169-172.
- Grewal, P.S., Ehlers, R.U. and Shapiro-Ilan, D.I. 2005. Critical issues and research needs for expanding the use of nematodes in biological control. In: Nematodes as biocontrol agents. Grewal, P.S., Ehlers, R.U. and Shapiro-Ilan, D.I. (eds.), CABI, Wallingford, UK, (pp. 479-489).
- Hamel, D., Rozman, V. and Liška, A. 2020. Storage of Cereals in Warehouses with or without Pesticides. Insects, 11 (12): 846.
- Javed, S., Khanum, T.A. and Khan, S. 2020. Biocontrol potential of entomopathogenic nematode species against Tribolium confusum (Jac.)(Coleoptera: Tenebrionidae) and Rhyzopertha dominica (Fab.)(Coleoptera: Bostrichidae) under laboratory conditions. Egyptian Journal of Biological Pest Control, 30: 1-6.
- Karan, S., Agrawal, N.S. and Girish, G.K. 1974. Studies on the quantitative loss in various high yielding varieties of maize, due to Sitophilus oryzae (L.) Col., Curculionidae. Labdev Journal of Science and Technology, 12 (1): 3-4.
- Kavallieratos, N.G., Athanassiou, C.G., Saitanis, C.J., Kontodimas, D.C., Roussos, A.N., Tsoutsa, M.S. and Anastassopoulou, U.A. 2007. Effect of two azadirachtin formulations against adults of Sitophilus oryzae and Tribolium confusum on different grain commodities. Journal of food protection, 70 (7): 1627-1632.
- Kunkel, B.A., Shapiro-Ilan, D.I., Campbell, J.F. and Lewis, E.E. 2006. Effect of Steinernema glaseri-infected host exudates on movement of conspecific infective juveniles. Journal of Invertebrate Pathology, 93 (1): 42-49.
- Laznik, Ž., Tóth, T., Lakatos, T., Vidrih, M. and Trdan, S. 2010. The activity of three new strains of Steinernema feltiae against adults of Sitophilus oryzae under laboratory conditions. Journal of Food, Agriculture and Environment, 8 (1): 150-154.
- Lunau, S., Stoessel, S., Schmidt-Peisker, A.J. and Ehlers, R.U. 1993. Establishment of monoxenic inocula for scaling up in vitro cultures of the entomopathogenic nematodes Steinernema spp. and Heterorhabditis spp. Nematologica, 39 (1-4): 385-399.
- Manosathiyadevan, M., Bhuvaneshwari, V. and Latha, R. 2017. Impact of insects and pests in loss of crop production: a review. Sustainable agriculture towards food security, 2017: 57-67.
- Muda, A.R. 1986. Pest problems and the use of pesticides in grain storage in Malaysia. ACIAR Proceedings Series, Australian Centre for International Agricultural Research, 1986: 11-16.
- Neethirajan, S., Karunakaran, C., Jayas, D.S. and White, N.D.G. 2007. Detection techniques for stored-product insects in grain. Food Control 18: 157-162.
- Negrisoli, C.R.D.C.B., Júnior, A.S.N., Bernardi, D. and Garcia, M.S. 2013. Activity of eight strains of entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) against five stored product pests. Experimental parasitology, 134 (3): 384-388.
- Oerke, E.C. and Dehne, H.W. 2004. Safeguarding production—losses in major crops and the role of crop protection. Crop protection, 23 (4): 275-285.
- Pereira, J.L., Antunes, S.C., Castro, B.B., Marques, C.R., Gonçalves, A.M., Gonçalves, F. and Pereira, R. 2009. Toxicity evaluation of three pesticides on non-target aquatic and soil organisms: commercial formulation versus active ingredient. Ecotoxicology, 18: 455-463.
- Rumbos, C.I. and Athanassiou, C.G. 2017. The use of entomopathogenic nematodes in the control of stored-product insects. Journal of Pest Science, 90: 39-49.
- Schöller, M., Flinn, P.W., Grieshop, M.J. and Zdarkova, E. 2006. Biological control of stored product pests. Insect management for food storage and processing, 2006: 67-87.
- Shapiro-Ilan, D.I., Gouge, D.H., Piggott, S.J. and Fife, J.P. 2006. Application technology and environmental considerations for use of entomopathogenic nematodes in biological control. Biological control, 38 (1): 124-133.
- Strauch, O. and Ehlers, R.U. 1998. Food signal production of Photorhabdus luminescens inducing the recovery of entomopathogenic nematodes Heterorhabditis spp. in liquid culture. Applied microbiology and biotechnology, 50: 369-374.
- Sunanda, B.S., Siddiqui, A.U. and Sharma, S. 2012. Effect of temperature on longevity of entomopathogenic nematodes, Steinernema abbasi and Heterorhabditis indica. Indian journal of nematology, 42 (1): 17-19.
- Susurluk, A. and Ehlers, R.U. 2008. Field persistence of the entomopathogenic nematode Heterorhabditis bacteriophora in different crops. BioControl, 53: 627-641.
- Susurluk, A., Şahin, Y.S. and Bouhari, A. 2018. Effects of some inorganic fertilizers on the entomopathogenic nematodes Steinernema feltiae (Tur-S3) and Heterorhabditis bacteriophora (HBH). Türkiye Biyolojik Mücadele Dergisi, 9 (2): 73-81.
- Swamy, K.N., Mutthuraju, G.P., Jagadeesh, E. and Thirumalaraju, G.T. 2014. Biology of Sitophilus oryzae (L.) (Coleoptera: Curculionidae) on stored maize grains. Current Biotica, 8 (1): 76-81.
- Şahin, Y.S., Boucharı, A., Ulu, T.C., Sadıç, B. and Susurluk, A. 2018. New application method for entomopathogenic nematode Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae) HBH strain against Locusta migratoria (Linnaeus, 1758) (Orthoptera: Acrididae). Turkish Journal of Entomology, 42 (4): 305-312.
- Trdan, S., Vidrih, M. and Valič, N. 2006. Activity of four entomopathogenic nematode species against young adults of Sitophilus granarius (Coleoptera: Curculionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae) under laboratory conditions/Wirkung von vier entomopathogenen Nematodenarten gegenüber Sitophilus granarius (Coleoptera: Curculionidae) und Oryzaephilus surinamensis (Coleoptera: Silvanidae) unter Laborbedingungen. Journal of plant diseases and protection, 2006: 168-173.
- Ulu, T.C., Sadic, B., Susurluk, I.A. and Aksit, T. 2015. Virulence of four entomopathogenic nematode species for plum sawfly, Hoplocampa flava L. (Hymenoptera: Tenthredinidae). Invertebrate Survival Journal, 12 (1): 274-277.
- Wouts, W.M. 1990. The primary form of Xenorhabdus species (Enterobacteriaceae: Eubacteriales) may consist of more than one bacterial species. Nematologica, 36 (1-4): 313-318.
- Zaller, J.G. and Brühl, C.A. 2019. Non-target effects of pesticides on organisms inhabiting agroecosystems. Frontiers in Environmental Science, 7: 75. doi: 10.3389/fenvs.2019.00075.
- Yüksel, E., Canhilal, R. and Imren, M. 2019. Potential of four Turkish isolates of entomopathogenic nematodes against three major stored products insect pests. Journal of Stored Products Research, 83: 317-321..
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Ziraat, Veterinerlik ve Gıda Bilimleri, Nematoloji |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | 219O370 |
| Erken Görünüm Tarihi | 24 Temmuz 2023 |
| Yayımlanma Tarihi | 23 Temmuz 2023 |
| Gönderilme Tarihi | 11 Mayıs 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 10 Sayı: 3 |
