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The effects of culture media and media components on the development of rat embryos

Year 2024, Volume: 8 Issue: 1, 75 - 81, 30.04.2024

İdil Özgenç Sema Birler

https://doi.org/10.30704/http-www-jivs-net.1452639

Abstract

After in vitro culture of rat embryos, blastocyst rates are lower than the other species because of the embryonic block observed in the 2- or 4-cell stages in vitro. Optimal culture media and systems that provide variable physiologic needs in the different stages of rat embryos. The modifications of rat embryo culture media could have a positive effect on increasing the blastocyst rates. However, since the results of rat embryo studies are changed depending on factors like strains preferred, maintenance conditions and different commercial products added to the culture media, the success rate of producing healthy newborns for reproductive biotechnological studies has not yet reached the desired level by using current embryo culture media. Understanding the needs of rat embryos cultured from zygote to blastocyst stage in vitro is important for successful advanced studies such as cloning and transgenesis. The purpose of this review is the effects of different culture media and media components on the preimplantation stages of rat embryos and get a perspective for developing the culture media

Keywords

culture media in vitro culture rat embryo

References

  • Agca Y. (2019). In vitro culture of rat preimplantation embryos.  In J. Herrick (Ed). Comparative Embryo Culture. Methods in Molecular Biology. vol 2006, 33-43, Humana, New York, NY.
  • Bavister, B. D. (1995). Culture of preimplantation embryos: facts and artifacts. Human reproduction update, 1(2), 91-148.
  • Berthois, Y, Katzenellenbogen, J. A, & Katzenellenbogen, B. S. (1986). Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proceedings of the National Academy of Sciences, 83(8), 2496-2500.
  • Biggers, J. D, Summers, M. C, & McGinnis, L. K. (1997). Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in culture media for mouse preimplantation embryos. Human Reproduction Update, 3(2), 125-135.
  • Brison, D. R, & Leese, H. J. (1991). Energy metabolism in late preimplantation rat embryos. Reproduction, 93(1), 245-251.
  • Ernst, M., Schmid, C., & Froesch, E. R. (1989). Phenol red mimics biological actions of estradiol: enhancement of osteoblast proliferation in vitro and of type I collagen gene expression in bone and uterus of rats in vivo. Journal of steroid biochemistry, 33(5), 907-914.
  • Gardner, D. K., & Lane, M. (1993). Amino acids and ammonium regulate mouse embryo development in culture. Biology of Reproduction, 48(2), 377-385.
  • Gómez, E, & Diez, C. (2000). Effects of glucose and protein sources on bovine embryo development in vitro. Animal Reproduction Science, 58(1-2), 23-37.
  • Han, M. S., & Niwa, K. (2003). Effects of BSA and fetal bovine serum in culture medium on development of rat embryos. Journal of Reproduction and Development, 49(3), 235-242.
  • Hickman, D. L., Johnson, J., Vemulapalli, T. H., Crisler, J. R., & Shepherd, R. (2017). Commonly used animal models. In M. A Suckow, K.L. Stewart (Ed). Principles of animal research for graduate and undergraduate students, 117.
  • Ho, Y., Wigglesworth, K., Eppig, J. J., & Schultz, R. M. (1995). Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Molecular reproduction and development, 41(2), 232-238.
  • Iannaccone, P., & Galat, V. (2014). Production of transgenic rats. In C.A. Pinkert (Ed). Transgenic animal technology 3th ed. (pp. 251-273). Elsevier.
  • Iannaccone, P., Taborn, G., & Garton, R. (2001). Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts. Zygote, 9(2), 135-143.
  • Kaneko, T., Kimura, S., & Nakagata, N. (2009). Importance of primary culture conditions for the development of rat ICSI embryos and long-term preservation of freeze-dried sperm. Cryobiology, 58(3), 293-297.
  • Kato, M., Ishikawa, A., Hochi, S., & Hirabayashi, M. (2004). Donor and recipient rat strains affect full-term development of one-cell zygotes cultured to morulae/blastocysts. Journal of Reproduction and Development, 50(2), 191-195.
  • Kishi, J., Noda, Y., Narimoto, K., Umaoka, Y., & Mori, T. (1991). Block to development in cultured rat 1-cell embryos is overcome using medium HECM-1. Human Reproduction, 6(10), 1445-1448.
  • Kito, S., Kaneko, Y., Yano, H., Tateno, S., & Ohta, Y. (2008). Developmental responses of 2-cell embryos to oxygen tension and bovine serum albumin in Wistar rats. Experimental Animals, 57(2), 123-128.
  • Kuran, M., Robinson, J. J, Staines, M. E, & McEvoy, T. G. (2001). Development and de novo protein synthetic activity of bovine embryos produced in vitro in different culture systems. Theriogenology, 55(2), 593-606.
  • Leese, H. J, McKeegan, P. J, & Sturmey, R. G. (2021). Amino acids and the early mammalian embryo: Origin, fate, function and life-long legacy. International Journal of Environmental Research and Public Health, 18(18), 9874.
  • Matsumoto, H., & Sugawara, S. (1995). Development of rat embryos at the 1-and 2-cell stage in modified HECM-1 medium after exposure to a medium that contained phosphate. Journal of Reproduction and Development, 41(4), 263-269.
  • Mayer, J. F., & Fritz, H. I. (1974). The culture of preimplantation rat embryos and the production of allophenic rats. Reproduction, 39(1), 1-9.
  • McKiernan, S. H, & Bavister, B. D. (1992). Different lots of bovine serum albumin inhibit or stimulate in vitro development of hamster embryos. In Vitro Cellular & Developmental Biology, 154-156.
  • Men, H., Amos-Landgraf, J. M., Bryda, E. C., & Franklin, C. L. (2023). KSOM-R supports both mouse and rat preimplantation embryo development in vitro. Theriogenology, 198, 69-74.
  • Men, H., Stone, B. J., & Bryda, E. C. (2020). Media optimization to promote rat embryonic development to the blastocyst stage in vitro. Theriogenology, 151, 81-85.
  • Miller, B. G, & Armstrong, D. T. (1981). Superovulatory doses of pregnant mare serum gonadotropin cause delayed implantation and infertility in immature rats. Biology of Reproduction, 25(2), 253-260.
  • Miyoshi, K. (2016). Development of a culture medium for rat 1-cell embryos. Journal of Mammalian Ova Research, 33(1), 11-16.
  • Miyoshi, K., & Niwa, K. (1997). Stage-specific requirement of phosphate for development of rat 1-cell embryos in a chemically defined medium. Zygote, 5(1), 67-73.
  • Miyoshi, K, Abeydeera, L. R, Okuda, K., & Niwa, K. (1995a). Effects of osmolarity and amino acids in a chemically defined medium on development of rat one-cell embryos. Reproduction, 103(1), 27-32.
  • Miyoshi, K., Funahashi, H., Okuda, K., & Niwa, K. (1994). Development of rat one-cell embryos in a chemically defined medium: effects of glucose, phosphate and osmolarity. Reproduction, 100(1), 21-26.
  • Miyoshi, K., Kono, T., & Niwa, K. (1997). Stage-dependent development of rat 1-cell embryos in a chemically defined medium after fertilization in vivo and in vitro. Biology of Reproduction, 56(1), 180-185.
  • Miyoshi, K., Tanaka, N., & Niwa, K. (1995b). Penetration in vitro of naturally ovulated rat eggs and the development of eggs in a chemically defined medium. Journal of Mammalian Ova Research, 12(1), 35-39.
  • Nakamura, K., Morimoto, K., Shima, K., Yoshimura, Y., Kazuki, Y., Suzuki, O., Matsuda, J., & Ohbayashi, T. (2016). The effect of supplementation of amino acids and taurine to modified KSOM culture medium on rat embryo development. Theriogenology, 86(8), 2083-2090.
  • Oh SH, Miyoshi K, & Funahashi H. (1998). Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage. Biology of reproduction, 59(4), 884-889.
  • Ohboshi S, Fujihara N, Yoshida T, & Tomogane H. (1998). Effects of glucose and amino acids on in-vitro development of rat preimplantation embryos, and morphological features of the developed blastocysts. Animal science and technology, 69, 637-645.
  • Popova E, Bader M, & Krivokharchenko A. (2011). Effect of culture conditions on viability of mouse and rat embryos developed in vitro. Genes, 2(2), 332-344.
  • Schini SA, & Bavister BD. (1988). Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biology of reproduction, 39(5), 1183-1192.
  • Seshagiri PB, & Vani V. (2019). Enabling hamster embryo culture system: development of preimplantation embryos. In J. Herrick (Ed). Comparative Embryo Culture: Methods and Protocols. vol 2006, 45-61, Humana, New York, NY.
  • Summers MC, & Biggers JD. (2003). Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues. Human reproduction update, 9(6), 557-582.
  • Telford NA, Watson AJ, & Schultz GA. (1990). Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Molecular reproduction and development, 26(1), 90-100.
  • Toyoda Y, & Chang MC. (1974). Fertilization of rat eggs in vitro by epididymal spermatozoa and the development of eggs following transfer. Reproduction, 36(1), 9-22.
  • Tsujii H, & Nakamura Y. (1999). Utilization of [U-14C] glucose by preimplantation rat embryo cultured in vitro. Journal of Mammalian Ova Research, 16(3), 94-97.
  • Vajta G, Rienzi L, Cobo A, & Yovich J. (2010). Embryo culture: can we perform better than nature?. Reproductive biomedicine online, 20(4), 453-469.
  • Yamada M, & Nishikimi A. (1999). Inhibitory effect of phosphate on in vitro development of 2-cell rat embryos is overcome by a factor (s) in oviductal extracts. FEBS letters, 462(1-2), 71-74.
  • Zhou Y, Galat V, Garton R, Taborn G, Niwa K, & Iannaccone P. (2003). Two‐phase chemically defined culture system for preimplantation rat embryos. genesis, 36(3), 129-133.

Year 2024, Volume: 8 Issue: 1, 75 - 81, 30.04.2024

İdil Özgenç Sema Birler

https://doi.org/10.30704/http-www-jivs-net.1452639

Abstract

References

  • Agca Y. (2019). In vitro culture of rat preimplantation embryos.  In J. Herrick (Ed). Comparative Embryo Culture. Methods in Molecular Biology. vol 2006, 33-43, Humana, New York, NY.
  • Bavister, B. D. (1995). Culture of preimplantation embryos: facts and artifacts. Human reproduction update, 1(2), 91-148.
  • Berthois, Y, Katzenellenbogen, J. A, & Katzenellenbogen, B. S. (1986). Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proceedings of the National Academy of Sciences, 83(8), 2496-2500.
  • Biggers, J. D, Summers, M. C, & McGinnis, L. K. (1997). Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in culture media for mouse preimplantation embryos. Human Reproduction Update, 3(2), 125-135.
  • Brison, D. R, & Leese, H. J. (1991). Energy metabolism in late preimplantation rat embryos. Reproduction, 93(1), 245-251.
  • Ernst, M., Schmid, C., & Froesch, E. R. (1989). Phenol red mimics biological actions of estradiol: enhancement of osteoblast proliferation in vitro and of type I collagen gene expression in bone and uterus of rats in vivo. Journal of steroid biochemistry, 33(5), 907-914.
  • Gardner, D. K., & Lane, M. (1993). Amino acids and ammonium regulate mouse embryo development in culture. Biology of Reproduction, 48(2), 377-385.
  • Gómez, E, & Diez, C. (2000). Effects of glucose and protein sources on bovine embryo development in vitro. Animal Reproduction Science, 58(1-2), 23-37.
  • Han, M. S., & Niwa, K. (2003). Effects of BSA and fetal bovine serum in culture medium on development of rat embryos. Journal of Reproduction and Development, 49(3), 235-242.
  • Hickman, D. L., Johnson, J., Vemulapalli, T. H., Crisler, J. R., & Shepherd, R. (2017). Commonly used animal models. In M. A Suckow, K.L. Stewart (Ed). Principles of animal research for graduate and undergraduate students, 117.
  • Ho, Y., Wigglesworth, K., Eppig, J. J., & Schultz, R. M. (1995). Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. Molecular reproduction and development, 41(2), 232-238.
  • Iannaccone, P., & Galat, V. (2014). Production of transgenic rats. In C.A. Pinkert (Ed). Transgenic animal technology 3th ed. (pp. 251-273). Elsevier.
  • Iannaccone, P., Taborn, G., & Garton, R. (2001). Preimplantation and postimplantation development of rat embryos cloned with cumulus cells and fibroblasts. Zygote, 9(2), 135-143.
  • Kaneko, T., Kimura, S., & Nakagata, N. (2009). Importance of primary culture conditions for the development of rat ICSI embryos and long-term preservation of freeze-dried sperm. Cryobiology, 58(3), 293-297.
  • Kato, M., Ishikawa, A., Hochi, S., & Hirabayashi, M. (2004). Donor and recipient rat strains affect full-term development of one-cell zygotes cultured to morulae/blastocysts. Journal of Reproduction and Development, 50(2), 191-195.
  • Kishi, J., Noda, Y., Narimoto, K., Umaoka, Y., & Mori, T. (1991). Block to development in cultured rat 1-cell embryos is overcome using medium HECM-1. Human Reproduction, 6(10), 1445-1448.
  • Kito, S., Kaneko, Y., Yano, H., Tateno, S., & Ohta, Y. (2008). Developmental responses of 2-cell embryos to oxygen tension and bovine serum albumin in Wistar rats. Experimental Animals, 57(2), 123-128.
  • Kuran, M., Robinson, J. J, Staines, M. E, & McEvoy, T. G. (2001). Development and de novo protein synthetic activity of bovine embryos produced in vitro in different culture systems. Theriogenology, 55(2), 593-606.
  • Leese, H. J, McKeegan, P. J, & Sturmey, R. G. (2021). Amino acids and the early mammalian embryo: Origin, fate, function and life-long legacy. International Journal of Environmental Research and Public Health, 18(18), 9874.
  • Matsumoto, H., & Sugawara, S. (1995). Development of rat embryos at the 1-and 2-cell stage in modified HECM-1 medium after exposure to a medium that contained phosphate. Journal of Reproduction and Development, 41(4), 263-269.
  • Mayer, J. F., & Fritz, H. I. (1974). The culture of preimplantation rat embryos and the production of allophenic rats. Reproduction, 39(1), 1-9.
  • McKiernan, S. H, & Bavister, B. D. (1992). Different lots of bovine serum albumin inhibit or stimulate in vitro development of hamster embryos. In Vitro Cellular & Developmental Biology, 154-156.
  • Men, H., Amos-Landgraf, J. M., Bryda, E. C., & Franklin, C. L. (2023). KSOM-R supports both mouse and rat preimplantation embryo development in vitro. Theriogenology, 198, 69-74.
  • Men, H., Stone, B. J., & Bryda, E. C. (2020). Media optimization to promote rat embryonic development to the blastocyst stage in vitro. Theriogenology, 151, 81-85.
  • Miller, B. G, & Armstrong, D. T. (1981). Superovulatory doses of pregnant mare serum gonadotropin cause delayed implantation and infertility in immature rats. Biology of Reproduction, 25(2), 253-260.
  • Miyoshi, K. (2016). Development of a culture medium for rat 1-cell embryos. Journal of Mammalian Ova Research, 33(1), 11-16.
  • Miyoshi, K., & Niwa, K. (1997). Stage-specific requirement of phosphate for development of rat 1-cell embryos in a chemically defined medium. Zygote, 5(1), 67-73.
  • Miyoshi, K, Abeydeera, L. R, Okuda, K., & Niwa, K. (1995a). Effects of osmolarity and amino acids in a chemically defined medium on development of rat one-cell embryos. Reproduction, 103(1), 27-32.
  • Miyoshi, K., Funahashi, H., Okuda, K., & Niwa, K. (1994). Development of rat one-cell embryos in a chemically defined medium: effects of glucose, phosphate and osmolarity. Reproduction, 100(1), 21-26.
  • Miyoshi, K., Kono, T., & Niwa, K. (1997). Stage-dependent development of rat 1-cell embryos in a chemically defined medium after fertilization in vivo and in vitro. Biology of Reproduction, 56(1), 180-185.
  • Miyoshi, K., Tanaka, N., & Niwa, K. (1995b). Penetration in vitro of naturally ovulated rat eggs and the development of eggs in a chemically defined medium. Journal of Mammalian Ova Research, 12(1), 35-39.
  • Nakamura, K., Morimoto, K., Shima, K., Yoshimura, Y., Kazuki, Y., Suzuki, O., Matsuda, J., & Ohbayashi, T. (2016). The effect of supplementation of amino acids and taurine to modified KSOM culture medium on rat embryo development. Theriogenology, 86(8), 2083-2090.
  • Oh SH, Miyoshi K, & Funahashi H. (1998). Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage. Biology of reproduction, 59(4), 884-889.
  • Ohboshi S, Fujihara N, Yoshida T, & Tomogane H. (1998). Effects of glucose and amino acids on in-vitro development of rat preimplantation embryos, and morphological features of the developed blastocysts. Animal science and technology, 69, 637-645.
  • Popova E, Bader M, & Krivokharchenko A. (2011). Effect of culture conditions on viability of mouse and rat embryos developed in vitro. Genes, 2(2), 332-344.
  • Schini SA, & Bavister BD. (1988). Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biology of reproduction, 39(5), 1183-1192.
  • Seshagiri PB, & Vani V. (2019). Enabling hamster embryo culture system: development of preimplantation embryos. In J. Herrick (Ed). Comparative Embryo Culture: Methods and Protocols. vol 2006, 45-61, Humana, New York, NY.
  • Summers MC, & Biggers JD. (2003). Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues. Human reproduction update, 9(6), 557-582.
  • Telford NA, Watson AJ, & Schultz GA. (1990). Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Molecular reproduction and development, 26(1), 90-100.
  • Toyoda Y, & Chang MC. (1974). Fertilization of rat eggs in vitro by epididymal spermatozoa and the development of eggs following transfer. Reproduction, 36(1), 9-22.
  • Tsujii H, & Nakamura Y. (1999). Utilization of [U-14C] glucose by preimplantation rat embryo cultured in vitro. Journal of Mammalian Ova Research, 16(3), 94-97.
  • Vajta G, Rienzi L, Cobo A, & Yovich J. (2010). Embryo culture: can we perform better than nature?. Reproductive biomedicine online, 20(4), 453-469.
  • Yamada M, & Nishikimi A. (1999). Inhibitory effect of phosphate on in vitro development of 2-cell rat embryos is overcome by a factor (s) in oviductal extracts. FEBS letters, 462(1-2), 71-74.
  • Zhou Y, Galat V, Garton R, Taborn G, Niwa K, & Iannaccone P. (2003). Two‐phase chemically defined culture system for preimplantation rat embryos. genesis, 36(3), 129-133.
There are 44 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences (Other)
Journal Section Review Articles
Authors

İdil Özgenç 0000-0001-5978-6042

Sema Birler 0000-0001-5069-5427

Publication Date April 30, 2024
Submission Date March 14, 2024
Acceptance Date April 2, 2024
Published in Issue Year 2024 Volume: 8 Issue: 1

Cite

APA Özgenç, İ., & Birler, S. (2024). The effects of culture media and media components on the development of rat embryos. Journal of Istanbul Veterinary Sciences, 8(1), 75-81. https://doi.org/10.30704/http-www-jivs-net.1452639

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