Research Article
Investigation of Phenolic Compounds and Antioxidant Capacity of Bee Pollen Collected from Different Geographical Regions in Turkey
Abstract
The aim of this study was to evaluate the antioxidan activity, total phenolic acid, and total flavonoid content in bee pollen collected from different regions of Turkey. A total of 81 bee pollen samples from 81 beekeepers were collected from 6 different regions of Turkey. Total phenolic content of pollen samples was determined in mg gallic acid equivalent gr pollen sample (mgGAE/gr) and total flavonoid analysis was made with a spectrophotometric method in mg based on quercetin (QE/g) concentration. Total antioxidant level was conducted according to free radical scavenger effects of pollen on DPPH (1,1-diphenyl-2-picrylhydrazyl) as an indirect method. According to the results of the study, total phenolic acid levels of pollen differed between 7.81 and 57.69 (mg GAE/g), total flavonoid content differed between 3.72 and 4.97 (mg QE/g) and DPPH differed between 55.18 and 94.08 (mg/ml). It is considered that the study data will contribute to the presentation of the chemical components of Turkish bee pollen.
Keywords
Supporting Institution
This work was supported by Ondokuz mayıs University, Scientific Research Projects Unit (BAP) under project number (PYO. VET.1904.16.005).
Project Number
(PYO. VET.1904.16.005)
Thanks
The authors thank Prof. Dr. Hatice Bozoglu for editing the content of this manuscript related to the statistical analysis.
References
- Almaraz-Abarca N, Campos M, Ávila-Reyes JA, Naranjo-Jiménez N, Corral JH, et. al. Antioxidant activity of polyphenolic extract of monofloral honeybee- collected pollen from mesquite. Journal of Food Composition and Analysis. 2007; 20: 119 –124.
- Asafova N, Orlov B, Kozin R. Physiologically active bee products. Y. A. Nikolaev, Nizhny Novgorod, Russia. edited by: Y. A. Nikolaev. 2001; 360-265.
- Avşar C, Özler H, Berber I, Civek S. Phenolic composition, antimicrobial and antioxidant activity of castaneasativa mill. pollen grains from Black Sea region of Turkey. International Food Research Journal. 2016; 23(4): 1711-1716.
- Aygul M, Yaylaci Karahalil F, Supuran CS. Investigation of the inhibitory properties of some phenolic standards and bee products against human carbonicanhydrase I and II. J. Enzyme Inhib Med Chem. 2016; 1-6.
- Bertoncelj J, Doberšek U, Jamnik M, Golob T. Evaluation of the phenolic content, antioxidant activity and colour of slovenian honey. Food Chemistry. 2007; 105: 822-828.
- Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002; 10(3): 178-182.
- Feas X, Vazquez-Tato MP, Estevinho L, Seijas JA, Iglesias A. Organic bee pollen botanical origin nutritional value bioactive compounds antioxidant activity and microbiological quality. Molecules. 2012; 17: 835-837.
- Freire KR, Lins AC, Dórea MC, Santos FA, Camara CA et.al. Palynological origin phenolic content and antioxidant properties of honeybee-collected pollen from bahia, Brazil. Molecules. 2012; 17:1652-1664.
- Graikou K, Kapeta S, Aligiannis N, Sotiroudis G, Chondrogianni N et.al. Chemical analysis of greek pollen - Antioxidant, antimicrobial and protea some activation properties. 2011;5 (33): 2-9.
- Kaškoniene V, Kaškonas P, Maruška A. Volatile compounds composition and antioxidant activity of bee pollen collected in Lithuania. Chemical Papers. 2015; 69(2): 291-299.
- Kroyer G, Hegedus N. Evaluation of bio active properties of pollen extracts as functional dietary food supplement. Innovative Food Science and Emerging Technologies. 2001; 2(3): 171-174.
- LeBlanc BW, Davis OK, Boue S, DeLucca A, Deeby T. Antioxidant activity of sonoran desert bee pollen. Food Chemistry. 2009; 115:1299-1305.
- Leja M, Mareczek A, Wyzgolik G, Klepacz Baniak J, Czekon´ska K. Antioxidative properties of bee pollen in selected plant species. Food Chemistry. 2007; 100: 237–240.
- Marghitas .A, Stanciu OG, Dezmirean DS, Bobis O, Popescu O et.al. Invitro antioxidant capacity of honey bee-collected pollen of selected floral origin harvested from Romania. Food Chemistry. 2009;115:878–883.
- Maruyama H, Sakamoto T, Araki Y, Hara H. Anti-inflammatory effect of bee pollen ethanol extract from cistus sp. of spanish on carrageenan induced rat hind paw edema. Complementary and Alternative Medicine. 2010; 10(30): 2-11.
- Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in burkina fasan honey, as well at their radical scavenging activity. Food Chem. 2005;91:571-577.
- Morais M, Moreira L, Feas, Estevinho LM. Honey bee collected pollen from five portuguese natural parks: palynological origin phenolic content antioxidant properties and antimicrobial activity. Food and Chemical Toxicology. 2011; 49:1096–1101.
- Nagai T, Inoue R, Inoue H, Suzuki N. Scavenging capacites of pollen extracts from cistus iadaniferus on autoxidation, superoxide radicals, hydroxyl radicals, and DPPH radicals. Nutition Research. 2002; 22: 519-526.
- Nisbet HÖ, Nisbet C, Yarım M, Güler A, Özak, A. Effects of different type of honey on healing of cutaneous wounds. Wounds. 2009; 21(7): 183-191.
- Pascoal A, Rodrigues S, Teixeira A, Feas X, Leticia M. Biological activities of commercial bee pollens: antimicrobial, antimutagenic, antioxidantand anti-inflammatory. Food and ChemicalToxicology. 2014; 63:233-239.
- Perez Pérez EM, Vit P, Rivas, E, Sciortino R, Sosa A et al. Antioxidant activity of four color fractions of bee pollen from Mérida, Venezuela. ArchLatinoam Nutr. 2012; 62(4): 375-80.
- Rebiai A, Lanez T. Chemical composition and antioxidant activity of apis mellifera bee pollen from north west Algeria. J.Fundam. Appl Sci. 2012; 4(2): 155-163.
- Silva TMS, Amorim Camara CA, Silva Linsa AC, Barbosa Filho JM, Silva EM et.al. Chemical composition and free radical scavenging activity of pollen loads from sting less bee Meliponasubnitida Ducke. Journal of Food Composition and Analysis. 2006; 19: 507-511.
- Tohamy AA, Abdella EM, Ahmed RR., Ahmed YK. Assessment of anti-mutagenic, anti-histopathologic and antioxidant capacities of egyptian bee pollen and propolis extracts. Cytotechnology. 2014; 66: 283-297.
- Ulusoy E. Determination of phenolic components of anzer honey and pollen by high performance liquid chromatography and their antioxidant properties. PhD thesis, Karadeniz Teknik university,Science Institute, Trabzon, 2010.
Türkiye'nin Farklı Coğrafi Bölgelerinden Toplanan Arı Poleninin Fenolik Bileşikleri ve Antioksidan Kapasitelerinin Araştırılması
Abstract
Bu çalışmada, Türkiye’nin değişik bölgelerinden toplanan balarısı polenlerin toplam fenolik asit, flavonoid düzeyleri ve total antioksidan kapasiteleri araştırılmıştır. Polen numuneleri Türkiye’nin farklı bölgelerden 81 arıcıdan toplam 81 örnekten temin edilmiştir. Toplam fenolik madde içeriği kuru ağırlığın gramı başına mg gallik asit eşdeğeri (mgGAE/gr) ve toplam flavonoid analizi ise gramı başına quercetin (mgQE/gr) konsantrasyon temel alınarak spektrofotometrik bir yöntem ile yapılmıştır. Total antioksidan düzeyi indirekt metot olarak polenin DPPH (1,1-diphenyl-2-picrylhydrazyl) üzerindeki serbest radikal süpürücü etkilerine göre yapılmıştır. Çalışmamızda elde edilen bulgulara göre polenin total fenolik asit düzeyleri 7.81 ile 57.69 (mgGAE/g), toplam flavonoid düzeyleri 3.72 ile 4.97 (mgQE/g) ve DPPH ise 55.18 ile 94.08 (mg/ml) aralığında değişkenlik göstermiştir. Çalışma verilerinin Türk arı poleninin kimyasal yapısının ortaya konulmasında katkı sağlayacağı düşünülmektedir.
Keywords
Project Number
(PYO. VET.1904.16.005)
References
- Almaraz-Abarca N, Campos M, Ávila-Reyes JA, Naranjo-Jiménez N, Corral JH, et. al. Antioxidant activity of polyphenolic extract of monofloral honeybee- collected pollen from mesquite. Journal of Food Composition and Analysis. 2007; 20: 119 –124.
- Asafova N, Orlov B, Kozin R. Physiologically active bee products. Y. A. Nikolaev, Nizhny Novgorod, Russia. edited by: Y. A. Nikolaev. 2001; 360-265.
- Avşar C, Özler H, Berber I, Civek S. Phenolic composition, antimicrobial and antioxidant activity of castaneasativa mill. pollen grains from Black Sea region of Turkey. International Food Research Journal. 2016; 23(4): 1711-1716.
- Aygul M, Yaylaci Karahalil F, Supuran CS. Investigation of the inhibitory properties of some phenolic standards and bee products against human carbonicanhydrase I and II. J. Enzyme Inhib Med Chem. 2016; 1-6.
- Bertoncelj J, Doberšek U, Jamnik M, Golob T. Evaluation of the phenolic content, antioxidant activity and colour of slovenian honey. Food Chemistry. 2007; 105: 822-828.
- Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis. 2002; 10(3): 178-182.
- Feas X, Vazquez-Tato MP, Estevinho L, Seijas JA, Iglesias A. Organic bee pollen botanical origin nutritional value bioactive compounds antioxidant activity and microbiological quality. Molecules. 2012; 17: 835-837.
- Freire KR, Lins AC, Dórea MC, Santos FA, Camara CA et.al. Palynological origin phenolic content and antioxidant properties of honeybee-collected pollen from bahia, Brazil. Molecules. 2012; 17:1652-1664.
- Graikou K, Kapeta S, Aligiannis N, Sotiroudis G, Chondrogianni N et.al. Chemical analysis of greek pollen - Antioxidant, antimicrobial and protea some activation properties. 2011;5 (33): 2-9.
- Kaškoniene V, Kaškonas P, Maruška A. Volatile compounds composition and antioxidant activity of bee pollen collected in Lithuania. Chemical Papers. 2015; 69(2): 291-299.
- Kroyer G, Hegedus N. Evaluation of bio active properties of pollen extracts as functional dietary food supplement. Innovative Food Science and Emerging Technologies. 2001; 2(3): 171-174.
- LeBlanc BW, Davis OK, Boue S, DeLucca A, Deeby T. Antioxidant activity of sonoran desert bee pollen. Food Chemistry. 2009; 115:1299-1305.
- Leja M, Mareczek A, Wyzgolik G, Klepacz Baniak J, Czekon´ska K. Antioxidative properties of bee pollen in selected plant species. Food Chemistry. 2007; 100: 237–240.
- Marghitas .A, Stanciu OG, Dezmirean DS, Bobis O, Popescu O et.al. Invitro antioxidant capacity of honey bee-collected pollen of selected floral origin harvested from Romania. Food Chemistry. 2009;115:878–883.
- Maruyama H, Sakamoto T, Araki Y, Hara H. Anti-inflammatory effect of bee pollen ethanol extract from cistus sp. of spanish on carrageenan induced rat hind paw edema. Complementary and Alternative Medicine. 2010; 10(30): 2-11.
- Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in burkina fasan honey, as well at their radical scavenging activity. Food Chem. 2005;91:571-577.
- Morais M, Moreira L, Feas, Estevinho LM. Honey bee collected pollen from five portuguese natural parks: palynological origin phenolic content antioxidant properties and antimicrobial activity. Food and Chemical Toxicology. 2011; 49:1096–1101.
- Nagai T, Inoue R, Inoue H, Suzuki N. Scavenging capacites of pollen extracts from cistus iadaniferus on autoxidation, superoxide radicals, hydroxyl radicals, and DPPH radicals. Nutition Research. 2002; 22: 519-526.
- Nisbet HÖ, Nisbet C, Yarım M, Güler A, Özak, A. Effects of different type of honey on healing of cutaneous wounds. Wounds. 2009; 21(7): 183-191.
- Pascoal A, Rodrigues S, Teixeira A, Feas X, Leticia M. Biological activities of commercial bee pollens: antimicrobial, antimutagenic, antioxidantand anti-inflammatory. Food and ChemicalToxicology. 2014; 63:233-239.
- Perez Pérez EM, Vit P, Rivas, E, Sciortino R, Sosa A et al. Antioxidant activity of four color fractions of bee pollen from Mérida, Venezuela. ArchLatinoam Nutr. 2012; 62(4): 375-80.
- Rebiai A, Lanez T. Chemical composition and antioxidant activity of apis mellifera bee pollen from north west Algeria. J.Fundam. Appl Sci. 2012; 4(2): 155-163.
- Silva TMS, Amorim Camara CA, Silva Linsa AC, Barbosa Filho JM, Silva EM et.al. Chemical composition and free radical scavenging activity of pollen loads from sting less bee Meliponasubnitida Ducke. Journal of Food Composition and Analysis. 2006; 19: 507-511.
- Tohamy AA, Abdella EM, Ahmed RR., Ahmed YK. Assessment of anti-mutagenic, anti-histopathologic and antioxidant capacities of egyptian bee pollen and propolis extracts. Cytotechnology. 2014; 66: 283-297.
- Ulusoy E. Determination of phenolic components of anzer honey and pollen by high performance liquid chromatography and their antioxidant properties. PhD thesis, Karadeniz Teknik university,Science Institute, Trabzon, 2010.
There are 25 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | RESEARCH ARTICLE |
| Authors | |
| Project Number | (PYO. VET.1904.16.005) |
| Publication Date | September 30, 2021 |
| Acceptance Date | August 25, 2021 |
| Published in Issue | Year 2021 Volume: 14 Issue: 3 |
Cite
Cited By
