Transcriptome and proteome analysis reveals the characteristics of carbon metabolism in the mycelial development of Gomphus purpuraceus

Gomphus purpuraceus is an ancient wild edible mushroom. However, the niche and lignocellulose degradation system of G. purpuraceus have remained cryptic. In the current study, transcriptome and proteome sequencing of G. purpuraceus in four mediums was performed. A total of 3489 differentially expressed genes (DEGs) and 891 differentially expressed proteins (DEPs) were screened out. For the lignocellulose degradation system, a total of 61 expressed genes were annotated, including 19 genes encoding cellulase, 8 genes encoding hemicellulase, 5 genes encoding pectinase, 22 genes encoding lignin oxidase, and 7 lignin degrading auxiliary enzyme. From two-omics, morphology, and enzyme activities, we speculated that G. purpuraceus might have a limited capacity to degrade polysaccharides (starch and lignocellulose). In addition, trace amount of fumonisins (FB1 and FB2) were found in the G. purpuraceus. These results provided a new perspective for understanding the key pathways and hub genes involved in carbon metabolism of G. purpuraceus development.