Production and optimization of Polyhydroxybutyrate and its application in bioplastic production

S.A. Kate¹ , D.R. Hire² , N.M. Pawar³

Section:Research Paper, Product Type: Isroset-Journal
Vol.6 , Issue.2 , pp.50-58, Apr-2019

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v6i2.5058

Online published on Apr 30, 2019

Copyright © S.A. Kate, D.R. Hire, N.M. Pawar . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Polyhydroxybutyrate (PHB) is a natural biopolymer by wide range of microbes which is a storage granule used by microbes as an energy as well as carbon source. PHB exhibit stiffness, brittle to rubber like, biodegradable, environment friendly properties. In present work isolates was produced Polyhydroxybutyrate by using Nitrogen deficient medium and modified LB medium with glucose as carbon source. Maximum production was observed in Modified LB medium as compared to nitrogen deficient medium. Extraction of bacterial PHB was done by sodium hypochlorite method; selected bacteria produced 2.10g/L PHB .Confirmation of PHB molecule was by FTIR & TLC. Extracted bacterial PHB was capable to produce biodegradable plastic which shows water tolerance activity. PHB production was found to be maximum at optimum pH 7.5, 37°C, 3% inoculums percentage and glucose concentration 20g/L.

Key-Words / Index Term :
PHB, Nitrogen deficient medium, Modified LB medium, FTIR, TLC, Biodegradable plastic

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