Assessment of Phenoloxidase Activity in Hemolymph of Pre, Inter and Post-Molting Stages

E. Angeline Christina Joybell¹ , Maduramozhi Govindarajalu²

1. P.G. and Research Department of Zoology, A.D.M. College for women (Autonomous) Velipalayam, Nagapattinam, India.
2. P.G. and Research Department of Zoology, A.D.M. College for women (Autonomous) Velipalayam, Nagapattinam, India.

Section:Research Paper, Product Type: Isroset-Journal
Vol.5 , Issue.5 , pp.80-82, Oct-2018

CrossRef-DOI:   https://doi.org/10.26438/ijsrbs/v5i5.8082

Online published on Oct 31, 2018

Copyright © E. Angeline Christina Joybell, Maduramozhi Govindarajalu . This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

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Abstract :
Phenoloxidase (PO) is an enzyme that vitally protects arthropods both internally and externally. PO protects arthropods internally from invasions by infectious microorganisms, and protects them externally by catalyzing the sclerotization of an animal’s newly formed or repaired exoskeleton In the present study determine the phenoloxidase activity in hemolymph of pre, inter and post molting stages. The phenoloxidase activity was highest in pre-molting followed by inter-molting and post-molting. The stability of the POs was very different depending on a number of factors such as temperatures, pH, substrate used, ionic strength, buffer system, and time of incubation. The results of the present study concluded that pre-molting stage has greater activity of phenol oxidase in hemolymph.

Key-Words / Index Term :
Phenoloxidase, Pre, Inter and Post molting stages, hemolymph

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