HPLC pigment concentrations from the Bermuda Atlantic Time-series Study (BATS) site from 1988-2022

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Date
2023-04-23
Authors
Johnson, Rodney J.
Bates, Nicholas
Lethaby, Paul J.
Medley, Claire
Smith, Dominic
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Date Created
2023-04-22
Location
Northwest Sargasso Sea at 31 deg 40' N, 64 deg 10' W
westlimit: -65.343; southlimit: 27.823; eastlimit: -62.718; northlimit: 33.142
DOI
10.26008/1912/bco-dmo.893521.1
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Keywords
BATS
HPLC pigments
phytoplankton pigments
algal pigments
Abstract
Data presented here contain HPLC derived phytoplankton pigments and fluorometric chlorophyll-a from the BATS site for years 1988 to 2022. Water samples are typically collected from 12 depths in the upper 250 meters of the water column, and then filtered under low vacuum through a 25mm GF/F filter. The filter is then flash frozen in liquid nitrogen and stored at -80 degrees C. Shoreside, analysis is performed on an HPLC using a method modified by Dr. R. Bidigare from the Wright et al. (1991) procedure. This method identifies the pigments chlorophyll-c3, chlorophyll-c2, peridinin, 19’-butanoyloxyfucoxanthin, fucoxanthin, 19’-hexanoyloxyfucoxanthin, prasinoxanthin, diadinoxanthin, alloxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll-b, chlorophyll-a, divinyl chlorophyllide-a, alpha and beta carotene. Additionally, chlorophyll-a and phaeopigments are analyzed using a fluorometric assay. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/893521
Description
Dataset: BATS HPLC pigments
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Citation
Johnson, R. J., Bates, N., Lethaby, P. J., Medley, C., & Smith, D. (2023). HPLC pigment concentrations from the Bermuda Atlantic Time-series Study (BATS) site from 1988-2022 (Version 1) [Data Set]. Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://doi.org/10.26008/1912/bco-dmo.893521.1
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