Electron Microscopic Cytochemical Analysis of Paracystalline Intranuclear Inclusions in Human Osteosarcoma Cells

FUMIOKI YASUZUMI; HIRONORI SAKAMOTO; YOSHIO MII; NORIKO YABUMOTO; GONPACHIRO YASUZUMI

doi:10.2535/ofaj1936.58.4-6_369

FUMIOKI YASUZUMI, HIRONORI SAKAMOTO, YOSHIO MII, NORIKO YABUMOTO, GONPACHIRO YASUZUMI

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Keywords: Human Osteosarcoma Cell, Intranuclear Inclusions, EDTA-Technique, X-ray Scanning Microanalysis, RNA

JOURNAL FREE ACCESS

1982 Volume 58 Issue 4-6 Pages 369-381

DOI https://doi.org/10.2535/ofaj1936.58.4-6_369

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Abstract

In order to study the ultrastructure and the chemical components of intranuclear paracrystalline inclusions appearing in human osteosarcoma cells, ultrathin sections were examined a trasmission electron microscope. The chemical properties of the inclusions were studied with perchloric acid, Bernhard's EDTA technique and X-ray scanning microanalysis. It was concluded that the intranuclear paracrystalline inclusions contain RNA.

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