Further experiments for developing the commercial cultivation of Pholiota adiposa were carried out. When the spawn-run was completed, the exhausted spawn on the substrate was removed with a raking machine (surface-clearance) . This treatment was essential for subsequent fruiting. Maintenance of sufficient moisture in the uppermost layer of the substrate was also required for fruiting. Therefore, the substrate was imbibed with water overnight just after the surface was cleared, after which polypropylene caps with side crevices were used again on the bottles until pins appeared. These treated bottles were then incubated at 18°C and 90-95% RH under dim light, and abundant pins appeared 12 days after surface-clearance. After the appearance of the pins, the caps were removed and the bottles were watered regularly twice a day with a sprinkler; normal development of fruit-bodies followed. Thus, the first pick was obtained within 26 days after surface-clearance.