Molecular Identification and Antibacterial Activity of Streptomyces spp. Isolated from Sulaymaniyah Governorate Soil, Iraqi Kurdistan

Syamand A. Qadir, Department of Medical Laboratory Science, Technical College of Applied Sciences, Sulaimani Polytechnic University, Halabja, Kurdistan Region, IraqFollow
Osama H. Shareef, Department of Medical Laboratory Science, Technical College of Applied Sciences, Sulaimani Polytechnic University, Halabja, Kurdistan Region, Iraq
Othman A. Mohammed, Department of Medical Laboratory Science, Technical College of Applied Sciences, Sulaimani Polytechnic University, Halabja, Kurdistan Region, Iraq

Corresponding Author

Syamand A. Qadir

Authors ORCID

0000-0002-6498-2643

Document Type

Research Article

Abstract

Soil is play an important role for reserve abundant groups of microorganisms, especially Streptomyces. Streptomyces are recognized as prokaryotes, aerobic and Gram-positive bacteria with high Guanine + Cytosine contents in their DNA. These groups of bacteria show filamentous growth from a single spore and they are normally found in all kinds of ecosystems, including water, soil, and plants. A total of three Streptomyces strains were isolated from soil of the sides of Darband Ranya in Sulaimani governorate. Different approaches were followed for the identification of the isolated stains. Morphological and cultural properties of these isolates have shown that the isolates are belonging to the genus Streptomyces. Desired colonies of the isolates were distinguished and separated from other bacteria on the basis of colony morphology, pigmentation, ability to produce a different color of aerial hyphae, and bottom mycelium on raffinose-histidine agar and starch-casein agar media. In addition, analysis of phylogenetic tree based on 16S rRNA gene sequences, the strains related the genus. KS010 isolates had the highest identity (99.32%) with the type strain of Streptomyces atrovirens, while KS005 and KS007 isolates were most closely related to Streptomyces lateritius by identity 99.32%. The isolated test strains were also active against Gram-negative and Gram-positive pathogenic bacteria following co-cultivation technique. However, further study should be done to identify the types of these antibacterial compounds

Keywords

16S rRNA, Antimicrobial activity, Genomic DNA, Streptomyces, Tyndallization technique

How to Cite This Article

Qadir, Syamand A.; Shareef, Osama H.; and Mohammed, Othman A. (2020) "Molecular Identification and Antibacterial Activity of Streptomyces spp. Isolated from Sulaymaniyah Governorate Soil, Iraqi Kurdistan," Polytechnic Journal: Vol. 10: Iss. 2, Article 18.
DOI: https://doi.org/10.25156/ptj.v10n2y2020.pp119-125