In order to reveal the NADH dehydrogenase subunit 5 (ND5) gene conformation of the Silky fowl, I designed 11 primers and sequenced the entire ND5 gene nucleotide sequences in the Black and White lines of Silky fowl, and compared it with that of White Leghorn chicken. The same nucleotide sequences were observed at ND5 gene region in the Black and White lines. The Silky fowl ND5 gene was transcribed from the H-strand of mitochondria DNA, consisted of 1, 818 by nucleotides (564A, 636C, 194G, and 424T) with no deletion and insertion, and encoded for a 605 amino acid protein. ND5 gene connected ND4 gene via three transfer RNA genes, tRNA (His), tRNA (Ser), and tRNA (Leu), at the 5' end, whereas at the 3' end it connected with a nucleotide sequence ACTA which is adjacent to cytochrome b (Cyt b) gene. Homologies of ND5 gene nucleotide sequences and amino acid sequences between the Silky fowl and White Leghorn chicken were 99.7% and 99.3%, respectively. The nucleotide sequence data reported in this article will appear in the EMBL, GenBank, and DDBJ nucleotide sequence data base under the accession number AF142407.