Pollen tube elongation in the styles of cross- and self-pollinated flowers of Japanese persimmon ‘Nishimurawase’ was investigated under 15°C, 25°C, and natural condition. Pollen tube lengths of ‘Nishimurawase’ and ‘Akagaki’ pollen in stylar sections (20μm), stained by aniline blue every 0.4mm from the stigma to top of ovule, were measured fluoroscopically. Almost no pollen germinated at 15°C. At 25°C, pollen tubes of ‘Akagaki’ elongated the length of the style within 24h after pollination, whereas, those of ‘Nishimurawase’ elongated to two-thirds of the length. The velocities of pollen tube growth were computed to be 1.0 and 0.6mm/h at 25°C for ‘Akagaki’ and ‘Nishimurawase’, respectively. Pollen tubes of ‘Akagaki’ were uniformly long, whereas, those of ‘Nishimurawase’ varied. The ability in pollen-tube elongation of ‘Nishimurawase’ pollen was less than that of ‘Akagaki’ pollen.