Journal of Hard Tissue Biology
Online ISSN : 1880-828X
Print ISSN : 1341-7649
ISSN-L : 1341-7649
Original
CD81 Mediated Regulation of Synoviolin Expression in Synovial Sarcoma Cells
Erika FujimotoYumi MatsushitaToshihiro NakajimaNaoko YagishitaTsutomu YamasakiTohru Nakanishi
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2016 Volume 25 Issue 4 Pages 377-382

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Abstract

Tetraspanin CD81, which is up-regulated in rheumatoid arthritis (RA) synoviocytes, is a molecule containing four transmembrane domains. We recently showed that small interfering RNA targeting CD81 (CD81 siRNA) ameliorated arthritis in rats. In that study, the expression of synoviolin was decreased in RA joints by CD81 siRNA. We also showed that CD81 siRNA decreased the expression of synoviolin and TNF-α in SW982 synovial sarcoma cells, suggesting that overexpression of CD81 in synovial cells induces the expression of both synoviolin and TNF-α in the same cells. Here we raised monoclonal antibodies (MAbs) against CD81 by immunization of the outer membrane region of CD81 (LEL) that was overexpressed as a recombinant protein. One of these MAbs stimulated CD81 on the membrane of SW982 and up-regulated the expression of synoviolin. We also defined the region of the synoviolin gene promoter that is essential for induction of synoviolin gene expression by using reporter gene system. This system might be useful for the screening of anti-RA medicine.

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© 2016 by The Hard Tissue Biology Network Association(JHTBNet)
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