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BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access December 3, 2011

Fibrinogen and cellular adherability on differently treated titanium as implants

  • Lukáš Fojt EMAIL logo , Petr Klapetek , Luděk Strašák and Vladimír Vetterl
From the journal Open Physics

Abstract

Adsorption of human plasma fibrinogen, osteoblasts, and fibroblasts on differently treated titanium samples as implants were examined in this study. Titanium samples were mechanically polished, chemically etched (with and without surface material loss), and grinded. The main goal of this study is to find the best surface treatment of titanium for its possible use as implants. Atomic force microscopy was used to evaluate the adsorption of human plasma fibrinogen onto the titanium samples. Cell counting was used to determine the adherability of osteoblasts and fibroblasts on the titanium samples. Our preliminary results show that the etched titanium surface with surface material loss is the best surface treatment used in our experiments.

[1] B. Walivaara, B. O. Aronsson, M. Rodahl, J. Lausmaa, P. Tengvall, Biomaterials 15, 827 (1994) http://dx.doi.org/10.1016/0142-9612(94)90038-810.1016/0142-9612(94)90038-8Search in Google Scholar

[2] V. Ball, A. Bentaleb, J. Hemmerle, J.-C. Voegel, P. Schaaf, Langmuir 12, 1614 (1996) http://dx.doi.org/10.1021/la950735v10.1021/la950735vSearch in Google Scholar

[3] N. Juany, P. Yang, Y. X. Leng, J. Y. Chen, H. Sun, J. Wang, G. J. Wang, P. D. Ding, T. F. Xi, Y. Leng, Biomaterials 24, 2177 (2003) http://dx.doi.org/10.1016/S0142-9612(03)00046-210.1016/S0142-9612(03)00046-2Search in Google Scholar

[4] K. Wang, Mat. Sci. Eng. A-Struct. 213, 134 (1996) http://dx.doi.org/10.1016/0921-5093(96)10243-410.1016/0921-5093(96)10243-4Search in Google Scholar

[5] M. V. Popa et al., Bioelectrochemistry 71, 126 (2007) http://dx.doi.org/10.1016/j.bioelechem.2007.02.00710.1016/j.bioelechem.2007.02.007Search in Google Scholar PubMed

[6] R. M. Souto, G. T. Burstein, J.Mater. Sci. 7, 337 (1996) http://dx.doi.org/10.1007/BF0015454510.1007/BF00154545Search in Google Scholar

[7] R. A. Gittens et al., Biomaterials 32, 3395 (2011) http://dx.doi.org/10.1016/j.biomaterials.2011.01.02910.1016/j.biomaterials.2011.01.029Search in Google Scholar PubMed PubMed Central

[8] P. Cacciafesta, A. D. L. Humphris, K. D. Jandt, M. J. Miles, Langmuir 16, 8167 (2000) http://dx.doi.org/10.1021/la000362k10.1021/la000362kSearch in Google Scholar

[9] R. Silvennoinen et al., Appl. Optics 49, 5583 (2010) http://dx.doi.org/10.1364/AO.49.00558310.1364/AO.49.005583Search in Google Scholar PubMed

[10] A. G. Hemmersam, M. Foss, J. Chevallier, F. Besenbacher, Colloid. Surface. B 43, 208 (2005) http://dx.doi.org/10.1016/j.colsurfb.2005.04.00710.1016/j.colsurfb.2005.04.007Search in Google Scholar PubMed

[11] I. van derKeere, R. Willaert, A. Hubin, J. Vereecken, Langmuir 24, 1844 (2008) http://dx.doi.org/10.1021/la701656610.1021/la7016566Search in Google Scholar

[12] K. Cai, J. Bossert, K. D. Jandt, Colloid. Surface. B 49, 136 (2006) http://dx.doi.org/10.1016/j.colsurfb.2006.02.01610.1016/j.colsurfb.2006.02.016Search in Google Scholar

[13] J. Y. Martin et al., J. Biomed. Mater. Res. 29, 389 (1995) http://dx.doi.org/10.1002/jbm.82029031410.1002/jbm.820290314Search in Google Scholar

[14] B. D. Boyan et al., Calcified Tissue Int. 71, 519 (2002) http://dx.doi.org/10.1007/s00223-001-1114-y10.1007/s00223-001-1114-ySearch in Google Scholar

[15] Y. T. Sul et al., Int. J. Oral Max. Impl. 20, 349 (2005) Search in Google Scholar

[16] D. Buser et al., J. Dent. Res. 83, 529 (2004) http://dx.doi.org/10.1177/15440591040830070410.1177/154405910408300704Search in Google Scholar

[17] P. Cacciafesta et al., Surf. Sci. 491, 405 (2001) http://dx.doi.org/10.1016/S0039-6028(01)01303-610.1016/S0039-6028(01)01303-6Search in Google Scholar

[18] A. Boukari, G. Francius, J. Hemmerle, J. Biomed. Mater. Res. A 78A, 466 (2006) http://dx.doi.org/10.1002/jbm.a.3077810.1002/jbm.a.30778Search in Google Scholar PubMed

[19] S. R. Sousa, M. M. Bras, P. Moradas-Ferreira, M. A. Barbosa, Langmuir 23, 7046 (2007) http://dx.doi.org/10.1021/la062956e10.1021/la062956eSearch in Google Scholar PubMed

[20] L. E. Averett, M. H. Schoenfisch, Analyst 135, 1201 (2010) http://dx.doi.org/10.1039/b924814e10.1039/b924814eSearch in Google Scholar PubMed

[21] Y. Estrin, C. Kasper, S. Diederichs, R. Lapovok, J. Biomed. Mater. Res. A 90A, 1239 (2009) http://dx.doi.org/10.1002/jbm.a.3217410.1002/jbm.a.32174Search in Google Scholar PubMed

[22] D. Franta, I. Ohlídal, P. Klapetek, Mikrochim. Acta 132, 443 (2000) http://dx.doi.org/10.1007/s00604005004210.1007/s006040050042Search in Google Scholar

[23] P. Dumas et al., Europhys. Lett. 22, 717 (1993) http://dx.doi.org/10.1209/0295-5075/22/9/01410.1209/0295-5075/22/9/014Search in Google Scholar

[24] P. Klapetek, I. Ohlídal, A. M. Ramil, A. Bonnanni, H. Sitter, Vacuum 80, 53 (2005) http://dx.doi.org/10.1016/j.vacuum.2005.07.02810.1016/j.vacuum.2005.07.028Search in Google Scholar

[25] R. Silvennoinen et al., Opt. Express 16, 10130 (2008) http://dx.doi.org/10.1364/OE.16.01013010.1364/OE.16.010130Search in Google Scholar PubMed

Published Online: 2011-12-3
Published in Print: 2012-2-1

© 2011 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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