The sterilization effect of three kinds of 0.01 -2.0 % (w/w) organic acid aqueous solutions (i.e. malic acid, succinic acid, citric acid) was evaluated to Saccharomyces cerevisiae NBRC 0308 at -80 -7℃. Consequently, the 0.5 -2.0% (w/w) of malic acid and citric acid solutions showed superior sterilization performance by processing for five days of -20℃. The survival rates of NBRC 0308 were 0.01% and less than 0.01%. On the other hand, with the 0.01-0.1% (w/w) solutions of malice acid and citric acid or the 0.01 -2.0% (w/w) succinic acid solution, the tendency that survival rates of NBRC 0308 decreased by processing for 5-10 days of 7℃ than -20℃ was observed.