Methylsterol components of Nervilia purpurea (I-tiam-hong), an Orchidaceous plant, were examined. The sterol fraction obtained from the neutral portion of the etherial extracts was purified by silica gel chromatography followed by preparative HPLC to give four new methylsterols, designated as cyclonervilasterol (1a), 24-epicyclonervilasterol (2a), dihydrocyclonervilasterol (3a), and 24-epidihydrocyclonervilasterol (4a), and their structures were determined based on the chemical and spectroscopic evidence. These sterols are unique in the structural feature having a cyclopropane ring at C_5-C_<10> positions. The stereochemistry at C_<24> position was assigned on the basis of the chemical shifts of the methyl groups in the ^1H-NMR spectra. Also the ^<13>C-NMR spectra were analysed in detail and the assignments of their carbon signals were done on the basis of INEPT and 2D-NMR methods.