マボヤにおけるハロシニンの生合成

渡辺 勝子; 仲村 和博; 山口 勝己; 鴻巣 章二

doi:10.2331/suisan.57.1587

Biosynthesis of Halocynine in the Ascidian Halocynthia roretzi

Katsuko Watanabe, Kazuhiro Nakamura, Katsumi Yamaguchi, Shoji Konosu

Author information

JOURNAL FREE ACCESS

1991 Volume 57 Issue 8 Pages 1587-1589

DOI https://doi.org/10.2331/suisan.57.1587

Details

Published: 1991 Received: March 12, 1991 Available on J-STAGE: February 29, 2008 Accepted: - Advance online publication: - Revised: -
Correction information
Date of correction: February 29, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : In order to know whether halocynine, a novel betaine discovered by us in the muscle of the ascidian Halocynthia roretzi, is endogenous or not, the incorporation of radioactivity into it was examined by injecting _L-(U-¹⁴C)lysine into the muscle of ascidian specimens. Halocynine isolated from the muscle extract by ion-exchange column chromatography and cellulose thin layer chro-matography was found to be radioactive in the specimen which had been kept in aerated sea water for 10 days after the administration of radioactive lysine. However, the isolated halocynine exhibited only a very weak activity and no activity in the specimens kept for 90 and 60h, respec-tively. These results unequivocally indicate that the ascidian is capable of synthesizing halocynine, although the biosynthetic rate is very slow under the conditions employed.
Right : In order to know whether halocynine, a novel betaine discovered by us in the muscle of the ascidian Halocynthia roretzi, is endogenous or not, the incorporation of radioactivity into it was examined by injecting L-(U-¹⁴C)lysine into the muscle of ascidian specimens. Halocynine isolated from the muscle extract by ion-exchange column chromatography and cellulose thin layer chro-matography was found to be radioactive in the specimen which had been kept in aerated sea water for 10 days after the administration of radioactive lysine. However, the isolated halocynine exhibited only a very weak activity and no activity in the specimens kept for 90 and 60h, respec-tively. These results unequivocally indicate that the ascidian is capable of synthesizing halocynine, although the biosynthetic rate is very slow under the conditions employed.

Date of correction: February 29, 2008 Reason for correction: - Correction: PDF FILE Details: -

Abstract

In order to know whether halocynine, a novel betaine discovered by us in the muscle of the ascidian Halocynthia roretzi, is endogenous or not, the incorporation of radioactivity into it was examined by injecting L-(U-¹⁴C)lysine into the muscle of ascidian specimens. Halocynine isolated from the muscle extract by ion-exchange column chromatography and cellulose thin layer chro-matography was found to be radioactive in the specimen which had been kept in aerated sea water for 10 days after the administration of radioactive lysine. However, the isolated halocynine exhibited only a very weak activity and no activity in the specimens kept for 90 and 60h, respec-tively. These results unequivocally indicate that the ascidian is capable of synthesizing halocynine, although the biosynthetic rate is very slow under the conditions employed.

Corresponding author

Correction information

Register with J-STAGE for free!