Zbornik Matice srpske za prirodne nauke 2011 Issue 120, Pages: 25-32
https://doi.org/10.2298/ZMSPN1120025M
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Elisa and HPLC analyses of deoxynivalenol in maize and wheat
Matić Jovana J. (Institut za tehnologiju hrane, Novi Sad)
Jajić Igor M. (Poljoprivredni fakultet, Departman za stočarstvo, Novi Sad)
Šarić Bojana M. (Institut za tehnologiju hrane, Novi Sad)
Mišan Aleksandra Č. (Institut za tehnologiju hrane, Novi Sad)
Krstović Saša Z. (Poljoprivredni fakultet, Departman za stočarstvo, Novi Sad)
Mandić Anamarija I. (Institut za tehnologiju hrane, Novi Sad)
Deoxynivalenol (DON) is a part of the family of mycotoxins called
trichothecenes which are produced by a number of different Fusarium mold
species. The presence of DON in 25 wheat and 25 maize samples was examined by
Enzyme Linked Immunosorbent Assay (ELISA) and High Performance Liquid
Chromatography (HPLC) methods. The presence of DON was detected and
determined in 5 (20%) maize and 6 (25%) wheat samples by both of the methods.
Correlation between ELISA and HPLC results was established, with the
correlation coefficients (r) of 0.9691 and 0.9735 for wheat and maize
samples, respectively. The results obtained by ELISA method were
significantly higher than those obtained by HPLC method. This fact can be
explained by the presence of conjugated or masked mycotoxins in the samples,
especially DON-3-glucoside (DON-3-Glc), which could not be determined by HPLC
method due to the lack of external standards. Contrary to this, being
insufficiently selective towards masked DON, ELISA method measures total DON
content of a sample. According to the obtained results, ELISA can be used as
a reliable screening method, but the confirmation of positive results must be
done by HPLC method.
Keywords: Deoxynivalenol, ELISA, HPLC, maize, wheat