Pesticidi i fitomedicina 2019 Volume 34, Issue 1, Pages: 61-67
https://doi.org/10.2298/PIF1901061C
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Assessment of genotoxic effects of a fungicide product and its active substances on human peripheral blood mononuclear cells
Cobanoglu Hayal (Çanakkale Onsekiz Mart University, Vocational Health College, Terzioglu Campus, Çanakkale, Turkey)
Coskun Baver (Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Animal Science, Terzioglu Campus, Çanakkale, Turkey)
Cayir Akin (Çanakkale Onsekiz Mart University, Vocational Health College, Terzioglu Campus, Çanakkale, Turkey)
The genotoxic potential of the plant protection product Signum® and its two
active substances (pyraclostrobin and boscalid) was investigated by the
comet assay (pH>13). Leukocytes isolated from whole blood were treated with
different concentrations (0.1-25 μg/ml) of the fungicide for 2 h and 20 h.
The Trypan Blue exclusion test showed higher cell viability (>84%) under all
three concentrations and in the two treatment periods. The obtained results
revealed that both Signum® and pyraclostrobin induced statistically
significant DNA damage. In contrast, boscalid did not cause statistically
significant DNA damage after 2 h exposure although it caused DNA damage at
higher concentrations after a longer time exposure (20 h). It is deducible
that pyraclostrobin and Signum® might be genotoxic. However, within the
studied concentration ranges, none of the fungicides was found to be
cytotoxic in the two treatment periods.
Keywords: genotoxicity, DNA damage, comet assay, fungicide, boscalid, pyraclostrobin