doiSerbia

Immobilization of β-glucosidase onto mesoporous silica support: Physical adsorption and covalent binding of enzyme

Ivetić Darjana Ž. (Faculty of Technology, Novi Sad)
Srdić Vladimir V. (Faculty of Technology, Novi Sad)
Antov Mirjana G. (Faculty of Technology, Novi Sad)

This paper investigates β-glucosidase immobilization onto mesoporous silica support by physical adsorption and covalent binding. The immobilization was carried out onto micro-size silica aggregates with the average pore size of 29 nm. During physical adsorption the highest yield of immobilized β-glucosidase was obtained at initial protein concentration of 0.9 mg ml-1. Addition of NaCl increased 1.7-fold, while Triton X-100 addition decreased 6-fold yield of adsorption in comparison to the one obtained without any addition. Covalently bonded β-glucosidase, via glutaraldehyde previously bonded to silanized silica, had higher yield of immobilized enzyme as well as higher activity and substrate affinity in comparison to the one physically adsorbed. Covalent binding did not considerably changed pH and temperature stability of obtained biocatalyst in range of values that are commonly used in reactions in comparison to unbounded enzyme. Furthermore, covalent binding provided biocatalyst which retained over 70% of its activity after 10 cycles of reuse.

Keywords: β-glucosidase, immobilization, physical adsorption, chemical binding, mesoporous silica

Projekat Ministarstva nauke Republike Srbije, br. III 45021