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HPLC-DAD of phenolics in bryophytes Lunularia cruciata, Brachytheciastrum velutinum and Kindbergia praelonga

Jocković Nebojša (Institute of Pharmaceutical Biology, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany)
Andrade Paula B. (Requimte, Institute of Pharmacognosy, Faculty of Pharmacy, University of Oporto, Porto, Portugal)
Valentão Patrícia (Requimte, Institute of Pharmacognosy, Faculty of Pharmacy, University of Oporto, Porto, Portugal)
Sabovljević Marko (Institut za botaniku i botanička bašta, Biološki fakultet, Beograd)

The chemistry of bryophytes is not well known. The available data indicate interesting chemical constitutions of some bryophyte species, i.e., active and new compounds are to be found within bryophytes, especially liverworts. In this study, one liverwort and two moss species were studied: Lunularia cruciata (L.) Dumort, Brachytheciastrum velutinum (Hedw) Ignatov & Huttunen and Kindbergia praelonga (Hedw) Ochyra. The phenolic compositions of these bryophyte species have not hitherto been reported. Their methanolic extracts were analyzed by reversed-phase HPLC, coupled to a diode-array detector (DAD). Luteolin-7-O-glucoside and quercetin were found in the L. cruciata extract. The extract obtained from B. velutinum contained four phenolic acids (4-O-caffeoylquinic, 5-O-caffeoylquinic, caffeic and ellagic acids) and three flavonoids (apigenin-7-O-glucoside, luteolin and apigenin). The K. praelonga extract was characterized by the presence of several phenolic acids and their derivatives (4-O-caffeoylquinic, 5-O-caffeoylquinic, caffeic, p-coumaric, ferulic and ellagic acids, and caffeic and p-coumaric acid derivatives) and three flavonoids (apigenin-7-O-glucoside, luteolin, apigenin and an unidentified flavanone). .

Keywords: bryophytes, phenolics, Lunularia cruciata, Brachytheciastrum velutinum, Kindbergia praelonga