Hemijska industrija 2015 Volume 69, Issue 2, Pages: 201-208
https://doi.org/10.2298/HEMIND130416009D
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Biosynthesis of components with antifungal activity against Aspergillus spp. using Streptomyces hygroscopicus
Dodić Jelena M. (Faculty of Technology, Novi Sad)
Rončević Zorana Z. (Faculty of Technology, Novi Sad)
Grahovac Jovana A. (Faculty of Technology, Novi Sad)
Bajić Bojana Ž. (Faculty of Technology, Novi Sad)
Korolija Olivera S. (Hemofarm a.d., Vršac)
Losses of apple fruit during storage are mainly caused by fungal
phytopathogens. Traditionally, postharvest fungal disease is controlled by
the application of synthetic fungicides. However, the harmful impact on
environment as well as human health largely limits their application. To
reduce these problems in agrochemicals usage, new compounds for plant
protection, which are eco-friendly, should be developed. The aim of this
study is optimization of medium composition in terms of glucose, soybean meal
and phosphates content, by applying response surface methodology, for the
production of agents with antifungal activity against Aspergillus spp. For
biosynthesis was used strain of Streptomyces hygroscopicus isolated from the
environment. Experiments were carried out in accordance with Box-Behnken
design with three factors on three levels and three repetitions in the
central point. Antifungal activity of the obtained cultivation mediums
against Aspergillus oryzae and Aspergillus niger was determined, in vitro,
using the diffusion - disc method. For determination optimal medium
components desirability function was used. Achieved model predicts that the
maximum inhibition zone diameter (40.93 mm) against test microorganisms is
produced when the initial content of glucose, soybean meal and phosphates are
47.77 g/l, 24.54 g/l and 0.98 g/l, respectively. To minimize the consumption
of medium components and costs of effluents processing, additional three sets
of optimization were made. The chosen method for optimization of medium
components was efficient, relatively simple and time and material saving.
Obtained results can be used for the further techno-economic analysis of the
process to select optimal medium composition for industrial application.
Keywords: Aspergillus spp., antifungal compounds, Streptomyces hygroscopicus, medium optimization