Genetika 2018 Volume 50, Issue 1, Pages: 69-84
https://doi.org/10.2298/GENSR1801069N
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Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites
Nikolić Biljana 
(Institute of Forestry, Belgrade)
Kovačević Dragan (Maize Research Institute “Zemun Polje”, Belgrade)
Mladenović-Drinić Snežana (Maize Research Institute “Zemun Polje”, Belgrade)
Nikolić Ana (Maize Research Institute “Zemun Polje”, Belgrade)
Mitić Zorica S. (Faculty of Sciences and Mathematics, Department of Biology and Ecology, Niš)
Bojović Srđan (Institute for Biological Research “Siniša Stanković” Belgrade)
Marin Petar D. (Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac“, Belgrade)
Genetic relationships among 12 taxa from subgenera Pinus and Strobus were
studied through fourteen microsatellite markers, previously developed for
Pinus taeda. To our knowledge, this is the first comparative study of pines
using nuclear EST-microsatellites (EST-SSRs). The total number of detected
alleles in all investigated taxa was 72 (5.14 in average). The numbers of
alleles per locus and PIC values for estimated markers ranged from 3 to 7,
and from 0.43 to 0.81, respectively. Presented results are in accordance
with majority of previous genetic investigations and infrageneric
classification of genus Pinus up to the sectional level, while subsectional
position of some species has still not dismissed, especially regarding
relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in
early-diverging position within subsection Pinaster and shows the greatest
closeness with P. halepensis, while Pinus peuce doesn't have basal position
within subsection Strobus being more close to P. strobus than to P.
wallichiana. Furthermore, the closest connections in subsection Pinus were
found between two Pinus nigra subspecies (dalmatica and nigra) as well as
between P. sylvestris and P. mugo.
Keywords: Bosnian pine, Macedonian pine, Austrian pine, nuclear EST-SSR markers
Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 173029 and Grant no. TR31068