Biotechnology in Animal Husbandry 2020 Volume 36, Issue 2, Pages: 215-223
https://doi.org/10.2298/BAH2002215K
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Expression of isg15 and conjugating enzyme during peri-implanation period in sheep
Khan Shahin Shah (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan)
Ali Haidar (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan)
Hayat Sher (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan)
Ahmad Sohail (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan)
Ibrahim Muhammad (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan)
Haider Syed Adnan (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan + Rehman Medical Institute Peshawar, KP Pakistan)
Ahmad Ijaz (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan)
Ul Haq Ihtesham (Еngineering, The University of Institute of Biotechnology and Genetic E Agriculture, Peshawar, KP Pakistan)
Pregnancy recognition in ruminant affects numerous genes regulation.
Interferon-stimulatory gene (ISG15), an ubiquitin-like protein that mediates
theconjugation of different proteins through its ISGylation enzymes UBE1L
and UBCH8, is also differentially expressed during early pregnancy. The
purpose of this study was to investigate the role of ISG-15 in the
establishment of pregnancy and conceptus elongation during early post
conception periods and to ascertain the presence of ISGylation enzymes UBE1L
and UBCH8. Therefore, sheep were synchronized through cloprostenol sodium
and gonadotrophin releasing hormone-1 and serviced by rams. The blood was
collected on the post-mating days 6, 11, 12, 13, 14, 16, 17, 21, 23 and 25.
The ISG-15, UBE1L and UBCH8 primers were used to amplify the corresponding
transcriptomic region using PCR. Recovery rate of each transcriptomic
fragment was compared with the housekeeping gene GAPDH. ISG-15 expression
was higher on day 12, contrary to UBE1L were higher on day 6 and UBCH8 on
day 21. Furthermore, the ISG-15 is ubiquitin-like protein, mediates UBE1L
and UBCH8 enzymes to guard the conceptus against viral pathogenicity during
early pregnancy.
Keywords: ISG-15, sheep, pregnancy, ISGylation, conception, ubiquitination