Acta Periodica Technologica 2014 Issue 45, Pages: 215-227
https://doi.org/10.2298/APT1445215P
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Chlorophyll a and chlorophyllide a inside liposomes made of saturated and unsaturated lipids: A possible impact of the lipids microenvironment
Petrović Sanja M. (Faculty of Technology, Leskovac)
Tugulea Laura (University of Bucharest, Faculty of Physics, Magurele, Romania)
Marković Dejan Z. (Faculty of Technology, Leskovac)
Barbanta-Patrascu Marcela (University of Bucharest, Faculty of Physics, Magurele, Romania)
The aim of this work was to examine a possible impact of liposomes lipids
microenvironment, dictated by a chemical composition of the fatty acid
branches, on incorporation and spectral behaviour of chlorophyll a, and its
derivative, chlorophyllide a inside small liposomes. The liposomes with the
incorporated chlorophylls were made of dimirystoyl phosphatidylcholine
(DMPC), and unsaturated phosphatidylcholine (PC), containing significant
fractions of unsaturated fatty acid moieties. In order to achieve the goal,
both absorption and fluorescence polarization spectroscopy were applied, and
the obtained data for the two incorporated pigments, which play a role of
molecular sensors, were compared. In addition, quercetin, a well-known
antioxidant, was used as the (chlorophylls) emission quencher, in order to
estimate the type of environment sensed by the two pigments for the two
liposomes that differ in chemical composition. The results, based primarily
on fluorescence polarization data have shown that the emissions as well as
the emission quenching were notably affected by a change in the lipids’
chemical composition. That is an indirect proof of the impact of the
liposomes microenvironment on the incorporated pigments’ spectral behaviour.
Keywords: chlorophyll, absorption, fluorescence, spectroscopy, liposomes
Projekat Ministarstva nauke Republike Srbije, br. TR-34012 i br. OI-172044