Acta Periodica Technologica 2014 Issue 45, Pages: 99-116
https://doi.org/10.2298/APT1445099V
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Antioxidant, antiproliferative and antimicrobial activity of freeze-dried raspberry
Vulić Jelena J. (Faculty of Technology, Novi Sad)
Velićanski Aleksandra S. (Faculty of Technology, Novi Sad)
Četojević-Simin Dragana D. (Oncology Institute of Vojvodina, Sremska Kamenica)
Tumbas-Šaponjac Vesna T. (Faculty of Technology, Novi Sad)
Đilas Sonja M. (Faculty of Technology, Novi Sad)
Cvetković Dragoljub D. (Faculty of Technology, Novi Sad)
Markov Siniša L. (Faculty of Technology, Novi Sad)
The main chemical composition, i.e. the total content of bioactive compounds
(phenolics 2209.86 ± 70.32 mg GAE/100g FDR, flavonoids 831.87 ± 12.61 mg
R/100g FDR and anthocyanins 144.55 ± 0.39 mg CGE/100g FDR), in freeze-dried
raspberry (FDR) was evaluated spectrophotometrically. Vitamin C content was
determined by HPLC analysis (88.81 ± 4.38 mg vit C/100g FDR). Antioxidant
activities of FDR extract were evaluated spectrophotometrically on stable
2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and by electron spin
resonance spectroscopy (ESR) method on hydroxyl radicals (•OH). EC50 values
were evaluated. EC50 DPPH• was 0.127 ± 0.013 mg/ml, while EC50 •OH was 1.366
± 0.026 mg/ml. Antiproliferative activity of the FDR extract was evaluated in
vitro in three human cell lines by colorimetric sulphorhodamine B (SRB)
assay. The most pronounced effects were obtained in the breast adenocarcinoma
cell line (MCF7). EC50 value was 395.07 ± 96.38 μg/ml. Antimicrobial activity
was determined by disk diffusion method. The FDR extract produced a clear
inhibition zone (without visible colonies) only toward Staphylococcus aureus.
The minimal inhibitory (MIC) and minimal bactericidal (MBC) concentrations of
FDR extract were evaluated. The values MIC were in the range of 4.7 - 100
mg/ml, and of MBC in the range of 6.3 - > 100 mg/ml.
Keywords: raspberry, freeze-drying, DPPH• and ESR antioxidant methods, antiproliferative and antimicrobial activity
Projekat Ministarstva
nauke Republike Srbije, br. TR 31044