Archives of Biological Sciences 2013 Volume 65, Issue 2, Pages: 595-602
https://doi.org/10.2298/ABS1302595A
Full text ( 1156 KB)
Cited by
Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers
Ahsyee Salem R. (El-Gabel El-Garbe University, Faculty of Natural Sciences, Tripoli, Libya)
Al-Sloge O. (Biology Research Centre, Tripoli, Libya)
Ćalić Irena (Faculty of Agriculture, Belgrade)
Branković Gordana (Faculty of Agriculture, Belgrade)
Zorić M. (Institute of Field and Vegetable Crops, Novi Sad)
Momirović Una (Faculty of Agriculture, Belgrade)
Vasiljević Sanja (Institute of Field and Vegetable Crops, Novi Sad)
Šurlan-Momirović Gordana (Faculty of Agriculture, Belgrade)
Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The
genetic diversity of nine alfalfa domesticated varietal populations was
studied using thirteen RAPD primer combinations. The number of polymorphic
fragments detected per primer combination ranged from 8 to 46 bands with an
average of 24 bands. The number of polymorphic bands detected was from 6
(Atalia population) to 37 (Gabsia population). The lowest genetic distance
was 0.058 and the highest was 0.655. The average genetic distance was
(0.356). The dendrogram based on Ward’s minimum variance clustering method
grouped the nine populations into the two main clusters. The first group
included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The
second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity
of RAPD assays for detection of genetic polymorphisms is confirmed in our
study, and results can be utilized in breeding practice.
Keywords: Alfalfa, genetic diversity, RAPD