Archives of Biological Sciences 2012 Volume 64, Issue 3, Pages: 877-883
https://doi.org/10.2298/ABS1203877T
Full text ( 692 KB)
Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia
Tasić S. (Department of Food Technology, Higher School of Applied Professional Studies, Vranje)
Kojić M. (Institute of Molecular Genetics and Genetic Engineering, Belgrade)
Stanković S. (Faculty of Biology, Belgrade)
Obradović D. (Institute of Food Technology and Biotechnology, Faculty of Agriculture, Belgrade)
The isolation and molecular characterization of bacterial strains isolated
from water sources in the Vlasina Mountain in southeast Serbia, confirmed the
presence of a new species Chryseobacterium vrystaatense ST1. This Gram-
negative species showed an extremely low level of biochemical reactivity in
biochemical tests. The gene for 16S rRNA was amplified by PCR using universal
primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic
features indicated that the isolate ST belonged to Chryseobacterium
vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA
gene with all sequences deposited in the NCBI collection showed the highest
similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov.,
designated as strain R-23533. The very high homology of these two strains
allowed classification of our strain at the species level, but some
differences indicate, and indirectly confirm, that the isolate ST is an
authentic representative. On the basis of these results, we could conclude
that Chryseobacterium vrystaatense ST was for first time isolated in Serbia,
which is particularly important when one bears in mind that there are only
three sequences of this species deposited in the NCBI collection.
Keywords: Chryseobacterium vrystaatense, 16S rRNA gene, oligomineral water