Archives of Biological Sciences 2008 Volume 60, Issue 4, Pages: 525-530
https://doi.org/10.2298/ABS0804525K
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PCR amplification and sequence analysis of the rat Sox3 gene
Krstić A. (Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd)
Mojsin Marija (Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd)
Kovačević-Grujičič Nataša (Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd)
Stevanović Milena 
(Institut za molekularnu genetiku i genetičko inženjerstvo, Beograd)
The Sox3 gene is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Despite the completion of a rat genome sequencing project, only a partial sequence of the rat Sox3 gene has been available in the public database. Using PCR, sequencing, and bioinformatics tools, in this study we have determined the complete coding sequence of the rat Sox3 gene encoding 449 amino acids. Comparative analysis of rat and human SOX3 proteins revealed a high degree of conservation. Identification of the rat Sox3 gene sequence would help in understanding the biological roles of this gene and provide insight into evolutionary relationships with vertebrate orthologs.
Keywords: Rattus norvegicus, laboratory rat, Sox3, comparative proteomics