Insertion/deletion polymorphism in the BRCA2 nuclear localization signal

Yasunaga YOSHIKAWA; Masami MORIMATSU; Kazuhiko OCHIAI; Masashi NAGANO; Yoshihisa YAMANE; Nobuyuki TOMIZAWA; Nobuo SASAKI; Kazuyoshi HASHIZUME

doi:10.2220/biomedres.26.109

Full Papers

Insertion/deletion polymorphism in the BRCA2 nuclear localization signal

Yasunaga YOSHIKAWA, Masami MORIMATSU, Kazuhiko OCHIAI, Masashi NAGANO, Yoshihisa YAMANE, Nobuyuki TOMIZAWA, Nobuo SASAKI, Kazuyoshi HASHIZUME

Author information

Yasunaga YOSHIKAWA
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
Laboratory of Cytology and Histology, Hokkaido University Graduate School of Medicine
Masami MORIMATSU
Laboratory of Cytology and Histology, Hokkaido University Graduate School of Medicine
Kazuhiko OCHIAI
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
Masashi NAGANO
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Yoshihisa YAMANE
Department of Veterinary Surgery, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Nobuyuki TOMIZAWA
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
Nobuo SASAKI
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
Kazuyoshi HASHIZUME
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University

JOURNAL FREE ACCESS

2005 Volume 26 Issue 3 Pages 109-116

DOI https://doi.org/10.2220/biomedres.26.109

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Abstract

Mutations in human BRCA2 confer an increased risk of female breast cancer. In this study, we found a novel insertion/deletion polymorphism (10204insAAA causing amino acid change M3332IK) in canine BRCA2, which is located in the putative second nuclear localization signal (NLS2) and C-terminal Rad51-binding region. The nuclear localization of the insAAA C-terminus was more efficient than localization of the delAAA sequence when NLS1 was mutated. Strong, comparable Rad51 binding was observed for both the insAAA and delAAA C-termini. Dogs with the insertion/deletion polymorphism will provide a new model for studying the function of BRCA2.

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