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Emergence of ISAba1 harboring carbapenem-resistant Acinetobacter baumannii isolates in Pakistan

    Mohsin Khurshid

    *Author for correspondence: Tel.: +92 333 430 1513;

    E-mail Address: mohsin.mic@gmail.com

    Department of Microbiology, Government College University, Faisalabad, Pakistan

    College of Allied Health Professionals, Directorate of Medical Sciences, Government College University, Faisalabad, Pakistan

    ,
    Muhammad Hidayat Rasool

    Department of Microbiology, Government College University, Faisalabad, Pakistan

    ,
    Usman Ali Ashfaq

    Department of Bioinformatics & Biotechnology, Government College University, Faisalabad, Pakistan

    ,
    Bilal Aslam

    Department of Microbiology, Government College University, Faisalabad, Pakistan

    &
    Muhammad Waseem

    Department of Microbiology, Government College University, Faisalabad, Pakistan

    Published Online:https://doi.org/10.2217/fmb-2017-0080

    Aim: The ISAba1 insertion sequence supports the overexpression of various oxacillinases genes by providing promoter and therefore, confers carbapenem resistance. The study aimed to determine the clonal diversity, genetic attributes of carbapenem resistance and the role of ISAba1 in the expression of oxacillinases genes among Acinetobacter baumannii clinical isolates. Materials & methods: PCR was performed to amplify various antimicrobial resistance determinants and ISAba1 insertion elements in 137 A. baumannii clinical isolates. Results: The blaOXA51 and blaOXA23-like genes and ISAba1 upstream to these genes were found in almost all multidrug-resistant isolates belonging to multiple clones. Conclusion: This is the first comprehensive study from Pakistan that highlighted the emergence of ISAba1 harboring carbapenems resistant A. baumannii stains in tertiary care hospitals.

    Graphical Abstract

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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