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Research Article

The 19q13 KRAB Zinc-finger protein ZFP82 suppresses the growth and invasion of esophageal carcinoma cells through inhibiting NF-κB transcription and inducing apoptosis

    Lin Ye

    Chongqing Key Laboratory of Molecular Oncology & Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China

    ,
    Tingxiu Xiang

    Chongqing Key Laboratory of Molecular Oncology & Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China

    ,
    Yu Fan

    Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou, PR China

    ,
    Dongsheng Zhang

    Cancer Epigenetics Laboratory, Department of Clinical Oncology, Sir YK Pao Center for Cancer & Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong & CUHK-Shenzhen Research Institute, Hong Kong, PR China

    Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060, PR China

    ,
    Lili Li

    Cancer Epigenetics Laboratory, Department of Clinical Oncology, Sir YK Pao Center for Cancer & Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong & CUHK-Shenzhen Research Institute, Hong Kong, PR China

    ,
    Chong Zhang

    Chongqing Key Laboratory of Molecular Oncology & Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China

    ,
    Xiaoqian He

    Chongqing Key Laboratory of Molecular Oncology & Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China

    ,
    Qin Xiang

    Chongqing Key Laboratory of Molecular Oncology & Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China

    ,
    Qian Tao

    *Author for correspondence: Tel.: +852 2632 1340; Fax: +852 2648 8842;

    E-mail Address: qtao@cuhk.edu.hk

    Chongqing Key Laboratory of Molecular Oncology & Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China

    Cancer Epigenetics Laboratory, Department of Clinical Oncology, Sir YK Pao Center for Cancer & Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong & CUHK-Shenzhen Research Institute, Hong Kong, PR China

    &
    Guosheng Ren

    **Author for correspondence: Tel.: +86 23 8901 2558; Fax: +86 23 8901 2558;

    E-mail Address: rengs726@126.com

    Chongqing Key Laboratory of Molecular Oncology & Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China

    Published Online:https://doi.org/10.2217/epi-2018-0092

    Aim: To study the epigenetic alternations and biological functions of ZFP82 in esophageal squamous cell carcinoma. Materials & methods: Analysis of ZFP82 expression was carried out by quantitative real-time PCR. Cell function was tested by MTS cell proliferation assay, transwell assay and flow cytometry. Gene mechanisms were studied by reverse transcription-PCR (RT-PCR), quantitative real-time PCR, luciferase reporter assay and Western blot.Results:ZFP82 promoter methylation was downregulated in esophageal squamous cell carcinoma. ZFP82 ectopic expression suppressed cell function and regulated NF-κB phosphorylation and genes involved in cell cycle arrest and apoptosis. Moreover, ZFP82 methylation was correlated with age, tumor stage and outcome. Conclusion:ZFP82 is a tumor suppressor and is disrupted by promoter CpG methylation during esophageal carcinogenesis.

    Papers of special note have been highlighted as: • of interest; •• of considerable interest

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