Antidiabetic Screening, Activity-guided Isolation and Molecular Docking Studies of Flower Extracts of Pongamia pinnata (L.) Pierre

Document Type : Research Paper

Authors

1 Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan

2 Department of Physics, University of Sargodha, Sargodha, Pakistan

Abstract

Pongamia pinnata (L.) Pierre (Family: Fabaceae) is a famous traditional medicinal plant, the flowers of which are used for treating diabetes, however, the active constituent(s) is yet unidentified. Therefore, the present study aimed to carry out antidiabetic activity-guided isolation of extracts of flowers. Hexane extract being the most active (EC50, 900 µg/mL) was fractionated by partitioning and the most active hexane fraction (EC50, 570 µg/mL) was subjected to column chromatography which gave three isomers of compound 1 (4-Methoxy-7-phenyl-5H-furo [3,2-g][1] benzopyran-5-one), a furanoflavonoid. All the isomeric forms have equal antidiabetic activity (83.24%) with EC50 at 300 µg/mL.  The activity of the isolated compound was found to be higher as compared to standard drug acarbose (43.46%). Molecular docking studies of the compound indicated higher binding energy scores with antidiabetic targets as compared to the standard drug acarbose. The results of the present study indicate that the isolated compound may be developed into an antidiabetic drug.

Keywords

References

1. Sangwan S, Rao DV, Sharma RA. A review on Pongamia pinnata (L.) Pierre: a great versatile leguminous plant. J Nat Sci. 2010;8:130-139.
2. Pulipati S, Babu PS, Lakshmi DN, Navyasri N, Harshini Y, Vyshnavi J, Prasanth M. A phyto pharmacological review on a versatile medicinal plant: Pongamia pinnata (L.) Pierre.  J Pharmacogn Phytochem. 2018;7:459-463.
3. Singh MP, Panda H. Medicinal herbs with their formulations, Daya Publishing House. 2005.
4. Warrier CRPK, Nambiar VPK. Indian medicinal plants: a compendium of 500 species, Orient Black Swan. 1993.s
5. Anuradha R, Krishnamoorthy P. Antioxidant activity of methanolic extract of Pongamia pinnata on lead acetate induced hepatic damage in rats. Afr J Biochem Res. 2011;2:348-351.
6. Babu DR, Rao GN. In vitro studies on extracts of Pongamia pinnata (L) Pierre flowers as a potent antioxidant. Int J Agric Food Sci Technol. 2010;1:7-11.
7. Kagithoju S, Godishala V, Pabba SK, Kurra H, Nanna RS. Antibacterial activity of flower extract of Pongamia pinnata Linn. an elite medicinal plant. Int J Pharm Pharm Sci. 2012;4:130-132.
8. Shirwaikar A, Malini S, Kumari SC. Protective effect of Pongamia pinnata flowers against cisplatin and gentamicin induced nephrotoxicity in rats. Indian J Exp Biol. 2003;41:58-62.
9. Chelvan VKK, Manavalan R, Balamurugan K, Ramanathan T. Hepatoprotective activity of Pongamia pinnata flower extract against Paracetamol induced hepatic damage in albino rats. Plant Arch. 2008;08:1023-1025.
10. Punitha R, Vasudevan K, Manoharan S. Effect of Pongamia pinnata flowers on blood glucose and oxidative stress in alloxan induced diabetic rats. Indian J Pharmacol. 2006;38:62-63.
11. Punitha, R, Manoharan S. Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats. J Ethnopharmacol. 2006;105:39-46.
12. Kumar P, Semalty A, Mir SR, Ali M, Amin S. Hypoglycemic and Hypolipidemic activity of Pongamia pinnata (Limn.) Pierre in Streptozotocin-induced Diabetic Rats. Int J Pharmacol. 2010;6:738-743.
13. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 2012;35:S64-71.
14. Luna B, Feinglos MN. Oral agents in the management of type 2 diabetes mellitus. Am Fam Physician. 2001;63:1747-56.
15. Mitri J, Hamdy O. Diabetes medications and body weight. Expert Opin. Drug Saf. 2009;8:573-584.
16. Gupta PD, De A. Diabetes Mellitus and its herbal treatment. Int J Pharm Biomed Sci. 2012;3:706-721.
17. Arumugam G, Manjula P, Paari N. A review: Anti diabetic medicinal plants used for diabetes mellitus. J Acute Dis. 2013;2:196-200.
18. Malviya N, Jain S, Malviya SAPNA. Antidiabetic potential of medicinal plants. Acta Pol Pharm. 2010;67:113-118.
19. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 1959;31:426-428.
20. Cirillo VP. Mechanism of glucose transport across the yeast cell membrane. J Bacteriol. 1962;84:485-91.
21. Bhutkar M, Bhise. In vitro hypoglycemic effects of Albizzia lebbeck and Mucuna pruriens. Asian Pac J Trop Biomed. 2013;3:866-870.
22. Adisa R, Choudhary M, Adewoye E, Olorunsogo O. Hypoglycaemic and biochemical properties of Cnestis ferruginea. Afr J Tradit Complement Altern Med. 2010;7:185-94.
23. Hanefeld M, Cagatay M, Petrowitsch T. Acarbose reduces the risk for myocardial infarction in type 2 diabetic patients: meta-analysis of seven longterm studies. Eur Heart J. 2004;25:10-16.
24. Chantrapromma S, Fun HK, Koysomboon S, Chantrapromma K. 4-Methoxy-7-phenyl-6, 7-dihydrofuro [3, 2-g] chromen-5-one. Acta Cryst E. 2006;62:o1984-o1986.
25. Rao RR, Tiwari AK, Reddy PP, Babu KS, Ali AZ, Madhusudana K, et al. New furanoflavanoids, intestinal α-glucosidase inhibitory and free-radical (DPPH) scavenging, activity from antihyperglycemic root extract of Derris indica (Lam.). Bioorgan Med Chem. 2009;17:5170-5175.
26. Das B, Chakravarty AK, Masuda K, Suzuki H, Ageta H. A diterpenoid from roots of Gelonium multiflorum. Phytochem. 1994;37:1363-1366.
27. Dai B, Dai X, Yang D, Qiu C. Chemical constituents of Fordia cauliflora (III). Zhong Cao Yao. 2003;34:1063-1065.
28. Pan S, Mukherjee B, Ganguly A, Mitra S, Bhattacharyya A. Antifungal activity of some naturally occurring flavonoids / Pilzhemmende Wirkung einiger natürlich vorkommender Flavonoide. Z Pflanz Bodenkunde/ J Plant Dis Protect. 1985;92:392-395.
29. Cazarolli LH, Folador P, Moresco HH, Brighente IMC, Pizzolatti MG, Silva FRMB. Stimulatory effect of apigenin-6-C-β-L-fucopyranoside on insulin secretion and glycogen synthesis. Eur J Med Chem. 2009;44:4668-4673.
30. Cazarolli LH, Pereira DF, Kappel VD, Folador P, Figueiredo MDSRB, Pizzolatti MG, Silva FRMB. Insulin signaling: a potential signaling pathway for the stimulatory effect of kaempferitrin on glucose uptake in skeletal muscle. Eur J Pharmacol. 2013;712:1-7.
31. Fang XK, Gao J, Zhu DN. Kaempferol and quercetin isolated from Euonymus alatus improve glucose uptake of 3T3-L1 cells without adipogenesis activity. Life Sci. 2008;82:615-622.
32. Zhang Y, Liu D. Flavonol kaempferol improves chronic hyperglycemia-impaired pancreatic beta-cell viability and insulin secretory function. Eur J Pharmacol. 2011;670:325-332.
33. Patra JC, Chua BH. Artificial neural network‐based drug design for diabetes mellitus using flavonoids. J Comput Chem. 2011;32:555-567.
34. Vinayagam R, Xu B. Antidiabetic properties of dietary flavonoids: a cellular mechanism review. Nutr Metab. 2015;12:60.
Journal of Medicinal plants and By-product
Volume 10, Issue 1
April 2021
Pages 85-92

Files

Share

How to cite

Statistics