اثر اسیدلینولئیک مزدوج و سدیم استات بر ماده خشک مصرفی، عملکرد و پروفایل چربی شیر در گاوهای هلشتاین تازهزا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران

چکیده

به منظور تعیین اثرات سدیم استات و اسید لینولئیک مزدوج (CLA) بر میزان ماده خشک مصرفی، قابلیت هضم خوراک، عملکرد و پروفایل چربی شیر، از 33 رأس گاو هلشتاین چند نوبت زایش طی روزهای 5-31 بعد زایش در قالب طرح کاملاً تصادفی با سه تیمار و 11 تکرار استفاده شد. جیره­های آزمایشی شامل 1-جیره پایه ، 2- جیره حاوی300 گرم مکمل سدیم استات، 3- جیره حاوی 100 گرم مکمل CLA بود. ماده خشک مصرفی و تولید شیر به­صورت روزانه، وزن بدن و نمره وضعیت بدنی در ابتدا و انتهای آزمایش اندازه گیری و نمونه شیر با فواصل منظم برای تعیین مقدار ترکیبات شیر گرفته شد. همچنین، در روز پایانی یک نمونه برای تعیین پروفایل اسیدهای چرب شبر گرفته شد. اختلاف معنی‌داری بین گروه دریافت­کننده سدیم استات و CLA با گروه شاهد برای ماده خشک مصرفی مشاهده نشد )05/0(p>. با مصرف سدیم استات و CLA تولید شیر به­ترتیب 16/3  و 46/2 کیلوگرم در روز نسبت به تیمار شاهد افزایش معنی­داری یافت. همان­طور که انتظار می­رفت مقدار چربی شیر با تیمار CLA کاهش داشت. با مصرف CLA توازن منفی انرژی تغییر معنی­دار نداشت که علت آن صرف انرژی ذخیره شده برای تولید شیر بیشتر می­باشد. مصرف CLA توانست با افزایش انتقال ایزومر ترانس 10 سیس 12 CLA به شیر و کاهش شاخص­های ترومبوژنیک و آتروزنیک برای سلامت مصرف کننده شیر مفید باشد. تولید و درصد پروتئین و لاکتوز شیر بین تیمارها تفاوت معنی­داری نداشت. عرضه استات سدیم باعث بهبود سنتز شیر و چربی شیر در گاوهای شیری شد، در نتیجه فرض لیپوژنیک بودن استات در بافت پستان تأیید شده است. در حالی­که، سدیم استات باعث منفی­تر کردن توازن منفی انرژی و افزایش شاخص آتروژنیک و ترومبوژنیک شد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of conjugated linoleic acid and sodium acetate on dry matter imtake, performance and milk fatty acid profile in Holstein cows

نویسندگان [English]

  • Sahar Karimi azandariani 1
  • Mahdi Ganjkhanlou 1
  • Kamran Rezayazdi 1
  • Abolfazl Zali 1
  • Hossein Moradi Shahrbabak 1
  • Mahdi Zhandi 1
  • Hamed Khalilvandi Behroozyar 2
1 Department of Animal Science, School of Agriculture and Natural Resources, University of Tehran, karaj, Iran
2 Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده [English]

In order to determine the effects of sodium acetate and conjugated linoleic acid (CLA) on dry matter consumed, feed digestibility, milk yield and milk fat profile, 33 Holstein cows several calving times during days 5-31 after calving in a completely randomized design with Three treatments and 11 replications were used. The experimental diets included 1- basic diet (control), 2- diet containing 300 gr of sodium acetate , 3- diet containing 100 gr of CLA. Daily dry matter intake and milk production, body weight and body condition score were measured at the beginning and end of the experiment. milk samples were taken at regular intervals to determine the amount of milk compounds. Also, on the last day, a sample was taken to determine the profile of fatty acids. Dry matter intake was not significant among dietary treatment (P>0.05). Milk production was increased by supplementing diets with sodium acetate (3.16 kg/d) and CLA (2.46 kg/d) compared to control treatment. CLA supplementing decreased milk fat content significantly and sodium acetate increased it. The yield and content of milk protein and lactose were not significantly different between the treatments. With the consumption of sodium acetate, the amount of milk fat and milk production increased, as probably the hypothesis that sodium acetate is lipogenic for adipose tissue was ignored and Acetate partition nutriunt toward milk fat production. CLA consumtion, negative energy balance did not change between as spared energy partition toward more milk yield. The use of CLA could be beneficial for the health of consumers by increasing the trans-10-cis-12 CLA isomer transfer to milk and reducing thrombogenic and atherogenic indicators.

کلیدواژه‌ها [English]

  • milk fat depression
  • biohydrogenation
  • milk fat synthesis
  • fatty liver
  • fat supplement

Extended Abstract

Introduction

In most cases, high-yielding cows are not able to provide the requierd energy in the early lactation period, and as a result, they are in a negative energy balance, which may lead to metabolic abnormalities and reproductive disorders. Adding conjugated linoleic acid (CLA) to the diet can reduce milk fat production in early lactation, thus leading to improved energy balance. Considering that acetate is a precursor for mammary lipogenesis in ruminants, it is logical that acetate can affect milk fat performance in lactating cows. The purpose of this experiment was to investigate the effect of CLA and sodium acetate supplementation on dry matter consumption, digestibility, milk production and milk fat profile in fresh cows.

 

Materias and methods

   This experiment was carried out in the educational-research station of the Department of Animal Science, Faculty of Agriculture, University of Tehran. 33 fresh Holstein cows were randomly assigned to 3 experimental treatments and 11 repetitions and were kept in individual stalls with free access to water during 5-31 days after parturition.

 

Results and discussion

   Sodium acetate did not improve the negative energy balance, and an increase in atherogenic and thrombogenic indices was also observed. While, the supply of sodium acetate improved the synthesis of milk and milk fat in dairy cows. The use of CLA could be beneficial for the health of milk consumers by increasing the transfer of trans-10-cis-12 isomer of CLA to milk and reducing thrombogenic and atherogenic indices. It seems that in the early parturition period, when the cows are in a negative energy balance, the use of sodium acetate is risky and the consumption of CLA is more rational.

Association of Official Analytical Chemists. (2000). Official Methods of Analysis. 17th ed. AOAC, washington, DC
Bauman, D.E., Griinari, J.M. (2003). Nutritional regulation of milk fat synthesis. Annual Review of Nutrition 23, 203-227.
Baumgard, L.H., Sangster, J.K., Bauman, D.E. (2001). Milk fat synthesis in dairy cows is progressively reduced by increasing supplemental amounts of trans-10, cis-12 conjugated linoleic acid (CLA). The Journal of Nutrition 131, 1764-1769.
Bernal-Santos, G., Perfield II, J., Barbano, D., Bauman, D., Overton, T.( 2003). Production responses of dairy cows to dietary supplementation with conjugated linoleic acid (CLA) during the transition period and early lactation. Journal of Dairy Science 86, 3218-3228.
Bjerre-Harpøth, V., Friggens, N.C., Thorup, V.M., Larsen, T., Damgaard, B., Ingvartsen, K., Moyes, K.(2012). Metabolic and production profiles of dairy cows in response to decreased nutrient density to increase physiological imbalance at different stages of lactation. Journal of Dairy Science 95, 2362-2380.
Castañeda-Gutiérrez, E., Benefield, B., De Veth, M., Santos, N., Gilbert, R., Butler, W., Bauman, D.(2007). Evaluation of the mechanism of action of conjugated linoleic acid isomers on reproduction in dairy cows. Journal of Dairy Science 90, 4253-4264.
Chitsaz, A., Ghorchi, T., H , S., S, F. (2011).The effect of protected conjugated linoleic acid supplementation on milk production, its composition and blood parameters in Holstein cows. Journal of Veterinary Research, 3.247-25.(In Persian)
Edmonson, A., Lean, I., Weaver, L., Farver, T., Webster, G.(1989). A body condition scoring chart for Holstein dairy cows. Journal of Dairy Science 72, 68-78.
Guliński, P.)2021(. Ketone bodies-causes and effects of their increased presence in cows' body fluids: A review. Veterinary world. 14 (6), 1492–1503.
Hötger, K., Hammon, H.M., Weber, C., Görs, S., Tröscher, A., Bruckmaier, R.M., Metges, C.C.(2013). Supplementation of conjugated linoleic acid in dairy cows reduces endogenous glucose production during early lactation. Journal of Dairy Science 96, 2258-2270.
Huang, Y., Schoonmaker, J., Bradford, B., Beitz, D.(2008). Response of milk fatty acid composition to dietary supplementation of soy oil, conjugated linoleic acid, or both. Journal of Dairy Science 91, 260-270.
Iwaniuk, M. E., Weidman, A. E., & Erdman, R. A. (2015). The effect of dietary cation-anion difference concentration and cation source on milk production and feed efficiency in lactating dairy cows. Journal of Dairy Science, 98(3), 1950-1960.
Mahdavi, A., Rezayazdi, K., Shahneh, A., Dehghan banadaki, M.(2010(. Studying the effect of conjugated linoleic acid on milk production and composition, energy balance and blood parameters of Holstein dairy cows in early lactation. Animal Science of  Iran.41. (In Persian)
Matamoros, C., Cai, J., Patterson, A., Harvatine, K.J.(2021). Comparison of the effects of short-term feeding of sodium acetate and sodium bicarbonate on milk fat production. Journal of Dairy Science 104, 7572-7582.
Maxin, G., Rulquin, H., Glasser, F.(2011). Response of milk fat concentration and yield to nutrient supply in dairy cows. Animal 5, 1299-1310.
Moore, C., Hafliger III, H., Mendivil, O., Sanders, S., Bauman, D., Baumgard, L.(2004). Increasing amounts of conjugated linoleic acid (CLA) progressively reduces milk fat synthesis immediately postpartum. Journal of Dairy Science 87, 1886-1895.
NRC. (2001). Nutrient Requirements of Dairy Cattle: 2001, National Academies Press.
Norman, H., Powell, R.(1999). Dairy cows of high genetic merit for yields of milk, fat and protein-Review. Asian-Australasian Journal of Animal Sciences 12, 1316-1323.
Odens, L., Burgos, R., Innocenti, M., VanBaale, M., Baumgard, L.(2007). Effects of varying doses of supplemental conjugated linoleic acid on production and energetic variables during the transition period. Journal of Dairy Science 90, 293-305.
Peterson, D., Kelsey, J., Bauman, D.(2002). Analysis of variation in cis-9, trans-11 conjugated linoleic acid (CLA) in milk fat of dairy cows. Journal of Dairy Science, 85, 2164-2172.
Roodbari, A.R., Towhidi, A., Zhandi, M., Rezayazdi, K., Mianji, G.R., Dirandeh, E., Colazo, M.(2016). Effect of conjugated linoleic acid supplementation during the transition period on plasma metabolites and productive and reproductive performances in dairy cows. Animal Feed Science and Technology 219, 294-303.
Schlegel, G., Ringseis, R., Windisch, W., Schwarz, F., Eder, K.(2012). Effects of a rumen-protected mixture of conjugated linoleic acids on hepatic expression of genes involved in lipid metabolism in dairy cows. Journal of Dairy Science 95, 3905-3918.
Selberg, K.T.(2004). Production and metabolic responses to dietary conjugated linoleic acid and trans-octadecenoic acid isomers in periparturient Holstein cows. Journal of Dairy Science 87, 158-168.
Sheikh, N., Zali, A., Ganjkhanlou, M., Towhidi, A., Dehghan, B,M.(2018). The effect of supplementary feeding of conjugated linoleic acid and a mixture of meal and sesame oil on yield and composition of milk fatty acids of Holstein dairy cows during the transition period. Iranian Journal of Veterinary Medicine.12,369-379. (In Persian)
Sheperd, A., Combs, D.(1998). Long-term effects of acetate and propionate on voluntary feed intake by midlactation cows. Journal of Dairy Science 81, 2240-2250.
Shingfield, K.J., Griinari, J.M.(2007). Role of biohydrogenation intermediates in milk fat depression. European Journal of Lipid Science and Technology 109, 799-816.
Urrutia, N., Bomberger, R., Matamoros, C., Harvatine, K.(2019). Effect of dietary supplementation of sodium acetate and calcium butyrate on milk fat synthesis in lactating dairy cows. Journal of Dairy Science 102, 5172-5181.
Urrutia, N., Harvatine, K.(2017a). Effect of conjugated linoleic acid and acetate on milk fat synthesis and adipose lipogenesis in lactating dairy cows. Journal of Dairy Science 100, 5792-5804.
Urrutia, N.L., Harvatine, K.J.(2017b). Acetate dose-dependently stimulates milk fat synthesis in lactating dairy cows. The Journal of Nutrition 147, 763-769.